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QuantSeq Expression Profiling Library Prep Kits

QuantSeq kits enable cost-efficient sequencing by counting. These kits are an exceptional alternative to standard RNA-Seq and microarrays. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple and accurate. By using Lexogen’s 96 i5 x 96 i7 dual indices up to 9,216 samples can be multiplexed in one lane, saving your sequencing space. Moreover, unique 96 i5 x 96 i7 indices help detect and quantify index hopping and minimize mis-assignment. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.

QuantSeq 3′ mRNA-Seq Library Prep Kit FWD is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail. The kit is available for Illumina and Ion Torrent platforms.

New! QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina is the most convenient solution for gene expression profiling for large screening projects. Using early pooling and batch processing it is easily scalable from a few to 36,864 samples.

QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.

QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.

New! Up to 384 Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output included in new QuantSeq kits (Cat. No. 113 – 115, 129 – 131).

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CORALL Total RNA-Seq Library Prep Kit

CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99%). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites.

New! Up to 384 Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output included in new CORALL kits (Cat. No. 117 – 119, 132 – 134).

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New! TraPR Small RNA Isolation Kit

The TraPR Small RNA Isolation Kit isolates functional, physiologically relevant silencing sRNAs from any organism, tissue, cell type, or bio-fluid. In contrast to previous state-of-the-art methods, this innovative 15-minute single-column workflow neither requires prior knowledge of the sample, nor does it involve tedious gel extraction steps or lengthy immuno-precipitation procedures. TraPR thus enables the extraction of high-quality sRNAs even from challenging or uncharacterized material, leading to highly reproducible sequencing results.

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Small RNA-Seq Library Prep Kit

Small RNA-Seq Library Prep Kit provides a protocol for generation of small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.

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SPLIT RNA Extraction Kit

SPLIT RNA Extraction Kit for extraction of total RNA or small and large RNA fractions free of genomic DNA from any cell type.

New! SPLIT RNA Extraction Kit for Blood for efficient depletion of globin mRNA during the extraction.

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Lexogen Indexing Solutions

Lexogen offers single and dual indexing solutions for all needs. Most common solutions are included in all Lexogen’s library prep kits and additional variations are available as Add-on Kits.

New! 384 Unique Dual Indices with superior error correction for maximal sequencing data output.

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SLAMseq Metabolic RNA Labeling Kit for RNA-Seq

SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics.

RiboCop rRNA Depletion Kits

RiboCop rRNA Depletion Kits for Human/Mouse/Rat and for Bacteria enable removal of ribosomal RNA from total RNA and are suited for Next Generation Sequencing as well as other demanding RNA analysis applications.

New! RiboCop HMR V2 with improved performance on degraded RNA samples.
New! Now also available for combined rRNA and globin mRNA depletion.

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TeloPrime Full-Length cDNA Amplification Kit V2

TeloPrime Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.

SIRVs product

SIRVs (Spike-in RNA Variant Control Mixes)

The SIRVs are a set of transcript variants designed to assess RNA sequencing workflows for their performance in transcriptome analysis in categories such as detection of isoforms, linearity of dose response and sensitivity, and transcript length. This includes the testing of bioinformatic algorithms for their ability to quantify, map, and assemble isoforms. In addition to these validation applications, the SIRVs can function as “fingerprints” to assess concordance of RNA-Seq data sets.

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Mix2 RNA-Seq Data Analysis Software

A software tool for the accurate estimation of RNA concentration from RNA-Seq data.

  • Precise transcript concentration estimates
  • Accurate detection of differential expression
  • Exceptional reproducibility across variable conditions
  • Fast run-times and small memory footprint
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SENSE mRNA-Seq Library Prep Kit

SENSETM mRNA-Seq Library Prep Kits generate ready-to-sequence libraries with exceptional strand-specificity from low amounts of input RNA within a few hours. SENSE mRNA-Seq Library Prep Kits are available for Illumina sequencing platforms. An automated SENSE mRNA-Seq Library Prep can be performed on the PerkinElmer Sciclone/Zephyr NGS workstations.

Library Prep Kits Overview

Kit Selection Criteria CORALL Total RNA-Seq SENSE mRNA QuantSeq Small RNA-Seq TeloPrime full-length cDNA
+ Poly(A) + RiboCop QuantSeq 3′ FWD QuantSeq 3′ REV QuantSeq-Flex
Expression Profiling yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon yes_icon no
Whole Transcriptome (full-transcript coverage) yes_icon yes_icon yes_icon yes_icon no no no no yes_icon
Alternative Polyadenylation / 3′ UTR isoforms no no no no yes_icon yes_icon* yes_icon no yes_icon
Isoform discovery & quantification yes_icon yes_icon* yes_icon* yes_icon no no yes_icon no yes_icon
Target-Specific no no no no no no yes_icon* no yes_icon
RiboSeq (Ribosomal profiling) no no no no no no no yes_icon* no
Polysome profiling no yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon no yes_icon
Transcript (re)annotation yes_icon yes_icon yes_icon yes_icon yes_icon 3’UTR only yes_icon 3’UTR only yes_icon yes_icon sRNA only yes_icon*
de novo assembly yes_icon yes_icon yes_icon yes_icon no no no no yes_icon*
SLAMseq (Nascent RNA-Seq) yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon yes_icon yes_icon
Small RNA  (e.g., miRNA) analysis no no no no no no no yes_icon* no
Long non-coding RNA analysis yes_icon yes_icon yes_icon yes_icon no no yes_icon no yes_icon
rRNA Sequencing yes_icon* no no no no no yes_icon no no
Total RNA yes_icon yes_icon* yes_icon* yes_icon yes_icon* yes_icon yes_icon yes_icon yes_icon
poly(A)+ RNA (≙ mRNA) yes_icon* no yes_icon no yes_icon yes_icon yes_icon no yes_icon
rRNA-depleted RNA yes_icon* yes_icon no no yes_icon yes_icon yes_icon no yes_icon
Small RNA no no no no no no no yes_icon* no
High quality (RIN ≥8) yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Moderate quality (RIN 5 – 8) yes_icon no yes_icon no yes_icon yes_icon yes_icon yes_icon no
FFPE / highly degraded (RIN <5; DV200 <50%) yes_icon M no yes_icon M no yes_icon M yes_icon M yes_icon M yes_icon no
Human, mouse, rat yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Other animals yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Plant yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Yeast yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Bacteria yes_icon no yes_icon no yes_icon pA yes_icon pA yes_icon yes_icon yes_icon
Virus yes_icon yes_icon pA yes_icon yes_icon pA yes_icon pA yes_icon pA yes_icon yes_icon yes_icon pA

(other sample types will also be compatible with most kits, please contact support@lexogen.com for further information)

Tissues, cell lines, primary or sorted cells etc. yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Blood yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon* yes_icon yes_icon yes_icon
Liquid biopsy (CSF, serum, plasma, urine) yes_icon no yes_icon no yes_icon M yes_icon yes_icon yes_icon* no
Exosomes yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon* no
Illumina yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Ion Torrent no no no no yes_icon no no no yes_icon
Oxford Nanopore no no no no no no no no yes_icon M
Pacific Biosciences no no no no no no no no yes_icon M
Single-read yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Paired-end yes_icon yes_icon yes_icon yes_icon no yes_icon yes_icon no yes_icon
Dual indexing compatible yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no
Automation-Compatible yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no no
UMIs available included included included no yes_icon no yes_icon no yes_icon
Data Analysis pipeline available yes_icon yes_icon yes_icon yes_icon included included yes_icon yes_icon yes_icon
Service available yes_icon yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon no
Spike In control recommendation SIRV-Set 1/2/3 SIRV-Set 3 no no SIRV-Set 1/2/3 yes_icon
8 rxn kit no no no yes_icon no no no yes_icon yes_icon
24 rxn kit yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
96 rxn kit yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no
2×96 rxn kit no no no no yes_icon yes_icon no no no
384 rxn kit no no no no yes_icon no no no no
Trial kit available (4 preps) yes_icon no yes_icon yes_icon yes_icon yes_icon no no yes_icon

Legend:

yes_icon* Optimal Choice
yes_icon Yes
yes_icon Possible, but not evaluated. For more information please contact support@lexogen.com.
yes_icon pA Possible only if a poly(A) tail (and a 5′ cap for TeloPrime) is present or with prior poly-adenylation
yes_icon M Protocol modifications apply
no Not possible or not useful