Product Overview
QuantSeq Expression Profiling Library Prep Kits
QuantSeq kits enable cost-efficient sequencing by counting. These kits are an exceptional alternative to standard RNA-Seq and microarrays. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple and accurate. By using Lexogen’s 96 i5 x 96 i7 dual indices up to 9,216 samples can be multiplexed in one lane, saving your sequencing space. Moreover, unique 96 i5 x 96 i7 indices help detect and quantify index hopping and minimize mis-assignment. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.
QuantSeq 3′ mRNA-Seq Library Prep Kit FWD is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail. The kit is available for Illumina and Ion Torrent platforms.
QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.
QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.
New! Globin Block Modules for QuantSeq are now available for human (Homo sapiens, Cat. No. 070.96) and pig (Sus scrofa, Cat. No. 071.96), enabling fully-integrated globin depletion for blood RNA-Seq library preparation, using the QuantSeq 3’ mRNA-Seq Library Prep protocol. The RS-Globin Block solution simply replaces the standard RNA Removal Solution, no additional steps are added to the protocol.
New! UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Cat. No. 081.96) contains the UMI Second Strand Synthesis Mix (USS). This mix simply replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq FWD Kit. The module allows unique tagging of individual transcripts with 6 nt long Unique Molecular Identifier (UMI) located between the partial P5 adapter and the random priming sequence. Use this module to identify PCR duplicates and eliminate amplification bias.
CORALL Total RNA-Seq Library Prep Kit
CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99%). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites.
SLAMseq Metabolic RNA Labeling Kit for RNA-Seq
SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics.
SIRVs (Spike-in RNA Variant Control Mixes)
The SIRVs are a set of transcript variants designed to validate the performance of isoform-specific RNA sequencing workflows, enabling bioinformatic algorithms to quantify, map, and assemble isoforms with exceptionally high accuracy.
Small RNA-Seq Library Prep Kit
Small RNA-Seq Library Prep Kit provides a protocol for generation of small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.
RiboCop rRNA Depletion Kit V1.3 (Human/Mouse/Rat)
RiboCop rRNA Depletion Kit V1.3 (Human/Mouse/Rat) enables removal of ribosomal RNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.
NEW! Faster protocol: rRNA depleted in only 1.5 hours (30 minutes hands-on time).
TeloPrime Full-Length cDNA Amplification Kit V2
TeloPrime Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.
SPLIT RNA Extraction Kit
SPLIT RNA Extraction Kit for extraction of total RNA or small and large RNA fractions free of genomic DNA from any cell type.
NEW! SPLIT RNA Extraction Kit for Blood for efficient depletion of globin mRNA during the extraction.
Lexogen Indexing Solutions
Lexogen offers single and dual indexing solutions for all needs. Most common solutions are included in all Lexogen’s library prep kits and additional variations are available as Add-on Kits.
NEW! 384 Unique Dual Indices with superior error correction for maximal sequencing data output.
Mix2 RNA-Seq Data Analysis Software
A software tool for the accurate estimation of RNA concentration from RNA-Seq data.
- Precise transcript concentration estimates
- Accurate detection of differential expression
- Exceptional reproducibility across variable conditions
- Fast run-times and small memory footprint
SENSE mRNA-Seq Library Prep Kit (SENSE Total RNA-Seq Library Prep Kit to be discontinued by 31.12.2019)
SENSE mRNA-Seq Library Prep Kit for fragmentation-free generation of RNA-Seq libraries with unprecedented strand-specificity and speed.
Library Prep Kits Overview
| Kit Selection Criteria | CORALL Total RNA-Seq | SENSE mRNA | QuantSeq | Small RNA-Seq | TeloPrime full-length cDNA | ||||
| + Poly(A) | + RiboCop | QuantSeq 3′ FWD | QuantSeq 3′ REV | QuantSeq-Flex | |||||
| Expression Profiling |  |
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| Whole Transcriptome (full-transcript coverage) | |
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| Alternative Polyadenylation / 3′ UTR isoforms | |
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| Isoform discovery & quantification | |
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| Target-Specific | |
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| RiboSeq (Ribosomal profiling) | |
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| Polysome profiling | |
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| Transcript (re)annotation | |
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3’UTR only |
3’UTR only |
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sRNA only |
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| de novo assembly | |
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| SLAMseq (Nascent RNA-Seq) | |
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| Small RNA (e.g., miRNA) analysis | |
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| Long non-coding RNA analysis | |
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| rRNA Sequencing | * |
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| Dual indexing compatible | |
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| Automation-Compatible | |
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| UMIs available | included | included | included | |
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| Data Analysis pipeline available | |
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included | included | |
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| Service available | |
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| Spike In control recommendation | SIRV-Set 1/2/3 | SIRV-Set 3 | |
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SIRV-Set 1/2/3 |
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| 8 rxn kit | |
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| 24 rxn kit | |
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| 96 rxn kit | |
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| 2×96 rxn kit | |
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| 384 rxn kit | |
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| Trial kit available (4 preps) | |
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Legend:
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Optimal Choice |
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Yes |
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Possible, but not evaluated. For more information please contact support@lexogen.com. |
| pA |
Possible only if a poly(A) tail (and a 5′ cap for TeloPrime) is present or with prior poly-adenylation |
| M |
Protocol modifications apply |
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Not possible or not useful |