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Product Overview

191.96-QuantSeq-UDIs-V2

QuantSeq kits enable cost-efficient RNA sequencing by counting. These kits are an exceptional alternative to standard whole-transcriptome RNA-Seq, microarrays or even qPCR. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple, fast, and accurate. By using Lexogen’s i5 and i7 dual indices, up to 9,216 or even 36,864 (QuantSeq-Pool) samples can be multiplexed in one lane, saving your sequencing space. Moreover, unique 96 i5 x 96 i7 indices help detect and quantify index hopping and minimize mis-assignment. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.

New! QuantSeq with UDI V2 takes you to the next level, offering increased robustness and compatibility with even the most challenging samples (FFPE, very low input), while keeping Lexogen’s patented 12nt Unique Dual Indices for optimal library identification and demultiplexing.

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New! Not sure which QuantSeq kit to use for your project?

Let us guide you with our QuantSeq Configurator Tool!

QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail.

QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina is the most convenient solution for gene expression profiling for large screening projects. Using early pooling and batch processing it is easily scalable from a few to 36,864 samples.

QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.

QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.

Up to 384 Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output included in new QuantSeq kits (Cat. No. 191 – 196).

The CORALL RNA-Seq Library Prep Kits enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome analyses using Illumina® NGS platforms. CORALL is the universal solution for all your samples offering exceptional performance on low input samples, down to 1 ng starting amount. It is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples.

New! CORALL RNA-Seq V2 allows generation of longer libraries without RNA fragmentation for demanding applications such as alternative splicing, isoform analysis, and fusion detection.

New! CORALL mRNA-Seq V2 Kits provide a complete solution for mRNA sequencing and include poly(A) selection and library preparation to generate sequencing-ready whole transcriptome mRNA libraries in 5.5 hours. All CORALL mRNA-Seq Kits contain Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output.

New! CORALL Total RNA-Seq V2 Kits are the ideal choice for Total RNA-Seq with ribo-depletion and for degraded and FFPE samples. For convenience, CORALL Total RNA-Seq V2 Kits are available as bundled versions with RiboCop rRNA Depletion Kits. All CORALL Total RNA-Seq V2 Kits and Bundles contain Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output.

221.24-LUTHOR-HD-3'mRNA-Seq-Kit-with-UDI

LUTHOR HD combines the novel THOR (THigh-resolution Original RNA amplification) technology with a highly efficient library preparation for 3’ mRNA-Seq analysis and is optimized for inputs of 1 to 100 cells, or 10 pg to 1 ng of extracted total RNA. This technology provides unmatched sensitivity and reproducibility and helps uncover the full transcriptomic status of the sample. This template-switch-, ligation-, and fragmentation-free protocol enables performing RNA-Seq even from challenging individual, singularized cells.

The TraPR Small RNA Isolation Kit isolates functional, physiologically relevant silencing sRNAs from any organism, tissue, cell type, or bio-fluid. In contrast to previous state-of-the-art methods, this innovative 15-minute single-column workflow neither requires prior knowledge of the sample, nor does it involve tedious gel extraction steps or lengthy immuno-precipitation procedures. TraPR thus enables the extraction of high-quality sRNAs even from challenging or uncharacterized material, leading to highly reproducible sequencing results.

Small RNA-Seq Library Prep Kit provides a protocol for generation of small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.

The SPLIT RNA Extraction Kit enables fast and highly efficient extraction of high quality, high purity RNA from a broad range of biological samples, including cell culture, animal and plant tissue, and fluid samples. Thus the obtained RNA is ideal for seamless library preparation for Next Generation Sequencing and other demanding applications. SPLIT recovers the complete RNA size ranges, including small RNAs.

101.96-UDI-12-nt-Index-Set

Lexogen offers single and dual indexing solutions for all needs. Most common solutions are included in all Lexogen’s library prep kits and additional variations are available as Add-on Kits.

384 Unique Dual Indices with superior error correction for maximal sequencing data output.

SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics.

RiboCop rRNA Depletion Kits for Human/Mouse/Rat for Bacteria, and Yeast enable removal of ribosomal RNA from total RNA and are suited for Next Generation Sequencing as well as other demanding RNA analysis applications.

New! RiboCop for Yeast. Why limit yourself to mRNA? Unlock the complete yeast transcriptome and analyze non-coding RNAs with RiboCop rRNA Depletion.

RiboCop HMR V2 with improved performance on degraded RNA samples.
Now also available for combined rRNA and globin mRNA depletion.

Lexogen’s RNA/DNA Defender Solution is a non-toxic storage solution that stabilizes and protects fragile RNA molecules in fresh tissue and cell culture samples. It eliminates the need to process samples immediately or freeze them in liquid nitrogen for later processing.

013.08-TeloPrime

TeloPrime Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.

025.03-SIRVs-Set-1

The SIRVs are a set of transcript variants designed to assess RNA sequencing workflows for their performance in transcriptome analysis in categories such as detection of isoforms, linearity of dose response and sensitivity, and transcript length. This includes the testing of bioinformatic algorithms for their ability to quantify, map, and assemble isoforms. In addition to these validation applications, the SIRVs can function as “fingerprints” to assess concordance of RNA-Seq data sets.

Library Prep Kits Overview

Kit Selection CriteriaCORALL Total RNA-SeqQuantSeqLUTHOR HD Single-Cell 3′ mRNA-SeqSmall RNA-SeqTeloPrime full-length cDNA
+ Poly(A)+ RiboCopQuantSeq 3′ FWD ★QuantSeq 3′ PoolQuantSeq 3′ REVQuantSeq-Flex
Expression Profiling

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Whole Transcriptome (full-transcript coverage)

Alternative Polyadenylation / 3′ UTR isoforms

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Isoform discovery & quantification

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Target-Specific

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RiboSeq (Ribosomal profiling)

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Polysome profiling

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Transcript (re)annotation

 3’UTR only

 3’UTR only

 3’UTR only

 3’UTR only

sRNA only

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de novo assembly

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SLAMseq (Nascent RNA-Seq)

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Small RNA (e.g., miRNA) analysis

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Long non-coding RNA analysis

rRNA Sequencing

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human or animal cells and protoplasts

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Total RNA

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poly(A)+ RNA (≙ mRNA)

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rRNA-depleted RNA

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Small RNA

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High quality (RIN ≥8)

Moderate quality (RIN 5 – 8)

FFPE / highly degraded (RIN <5; DV200 <50%)

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Low Input <100 pg

Human, mouse, rat

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Other animals

Plant

Yeast

Bacteria

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Virus

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Tissues, cell lines, primary or sorted cells etc.

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Blood

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Liquid biopsy (CSF, serum, plasma, urine)

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Exosomes

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Illumina

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Oxford Nanopore

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Pacific Biosciences

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Single-read

Paired-end

Dual indexing compatible

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Automation-Compatible

UMIs availableincludedincludedincluded

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Data Analysis pipeline available

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included

Service available

Spike In control recommendationSIRV-Set 1/2/3SIRV-Set 3

SIRV-Set 3

SIRV-Set 1/2/3

8 rxn kit

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24 rxn kit

96 rxn kit

2×96 rxn kit

384 rxn kit

Product Configurator

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Legend:

QuantSeq FWD also exists in a bundle package as “QuantSeq with UDI V2 Kit” (recommended option)

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Optimal Choice

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Yes

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Possible, but not evaluated. For more information please contact support@lexogen.com.

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Possible only if a poly(A) tail (and a 5′ cap for TeloPrime) is present or with prior poly-adenylation

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Protocol modifications apply

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Not possible or not useful

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