Product Overview

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QuantSeq Expression Profiling Library Prep Kits

QuantSeq kits enable cost-efficient sequencing by counting. These kits are an exceptional alternative to standard RNA-Seq and microarrays. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple and accurate. By using Lexogen’s 96 i5 x 96 i7 dual indices up to 9,216 samples can be multiplexed in one lane, saving your sequencing space. Moreover, unique 96 i5 x 96 i7 indices help detect and quantify index hopping and minimize mis-assignment. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.

QuantSeq 3′ mRNA-Seq Library Prep Kit FWD is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail. The kit is available for Illumina and Ion Torrent platforms.

QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.

QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.

New! Globin Block Modules for QuantSeq are now available for human (Homo sapiens, Cat. No. 070.96) and pig (Sus scrofa, Cat. No. 071.96), enabling fully-integrated globin depletion for blood RNA-Seq library preparation, using the QuantSeq 3’ mRNA-Seq Library Prep protocol. The RS-Globin Block solution simply replaces the standard RNA Removal Solution, no additional steps are added to the protocol.

New! UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Cat. No. 081.96) contains the UMI Second Strand Synthesis Mix (USS). This mix simply replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq FWD Kit. The module allows unique tagging of individual transcripts with 6 nt long Unique Molecular Identifier (UMI) located between the partial P5 adapter and the random priming sequence. Use this module to identify PCR duplicates and eliminate amplification bias.

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CORALL Total RNA-Seq Library Prep Kit

CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99%). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites.

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SLAMseq Metabolic RNA Labeling Kit for RNA-Seq

SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics.

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SIRVs (Spike-in RNA Variant Control Mixes)

The SIRVs are a set of transcript variants designed to validate the performance of isoform-specific RNA sequencing workflows, enabling bioinformatic algorithms to quantify, map, and assemble isoforms with exceptionally high accuracy.

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Small RNA-Seq Library Prep Kit

Small RNA-Seq Library Prep Kit provides a protocol for generation of small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.

RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat)

RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat) enables removal of ribosomal RNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.

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TeloPrime Full-Length cDNA Amplification Kit V2

TeloPrime Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.

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SPLIT RNA Extraction Kit

SPLIT RNA Extraction Kit for extraction of total RNA or small and large RNA fractions free of genomic DNA from any cell type.

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Mix2 RNA-Seq Data Analysis Software

A software tool for the accurate estimation of RNA concentration from RNA-Seq data.

  • Precise transcript concentration estimates
  • Accurate detection of differential expression
  • Exceptional reproducibility across variable conditions
  • Fast run-times and small memory footprint
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SENSE mRNA-Seq Library Prep Kit (SENSE Total RNA-Seq Library Prep Kit to be discontinued by 31.12.2019)

SENSE mRNA-Seq Library Prep Kit for fragmentation-free generation of RNA-Seq libraries with unprecedented strand-specificity and speed.

Library Prep Kits Overview

Kit Selection Criteria CORALL Total RNA-Seq SENSE mRNA QuantSeq Small RNA-Seq TeloPrime full-length cDNA
+ Poly(A) + RiboCop QuantSeq 3′ FWD QuantSeq 3′ REV QuantSeq-Flex
Expression Profiling yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon yes_icon no
Whole Transcriptome (full-transcript coverage) yes_icon yes_icon yes_icon yes_icon no no no no yes_icon
Alternative Polyadenylation / 3′ UTR isoforms no no no no yes_icon yes_icon* yes_icon no yes_icon
Isoform discovery & quantification yes_icon yes_icon* yes_icon* yes_icon no no yes_icon no yes_icon
Target-Specific no no no no no no yes_icon* no yes_icon
RiboSeq (Ribosomal profiling) no no no no no no no yes_icon* no
Polysome profiling no yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon no yes_icon
Transcript (re)annotation yes_icon yes_icon yes_icon yes_icon yes_icon 3’UTR only yes_icon 3’UTR only yes_icon yes_icon sRNA only yes_icon*
de novo assembly yes_icon yes_icon yes_icon yes_icon no no no no yes_icon*
SLAMseq (Nascent RNA-Seq) yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon yes_icon yes_icon yes_icon
Small RNA  (e.g., miRNA) analysis no no no no no no no yes_icon* no
Long non-coding RNA analysis yes_icon yes_icon yes_icon yes_icon no no yes_icon no yes_icon
rRNA Sequencing yes_icon* no no no no no yes_icon no no
Total RNA yes_icon yes_icon* yes_icon* yes_icon yes_icon* yes_icon yes_icon yes_icon yes_icon
poly(A)+ RNA (≙ mRNA) yes_icon* no yes_icon no yes_icon yes_icon yes_icon no yes_icon
rRNA-depleted RNA yes_icon* yes_icon no no yes_icon yes_icon yes_icon no yes_icon
Small RNA no no no no no no no yes_icon* no
High quality (RIN ≥8) yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Moderate quality (RIN 5 – 8) yes_icon no yes_icon no yes_icon yes_icon yes_icon yes_icon no
FFPE / highly degraded (RIN <5; DV200 <50%) yes_icon M no yes_icon M no yes_icon M yes_icon M yes_icon M yes_icon no
Human, mouse, rat yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Other animals yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Plant yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Yeast yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Bacteria yes_icon no yes_icon no yes_icon pA yes_icon pA yes_icon yes_icon yes_icon
Virus yes_icon yes_icon pA yes_icon yes_icon pA yes_icon pA yes_icon pA yes_icon yes_icon yes_icon pA

(other sample types will also be compatible with most kits, please contact support@lexogen.com for further information)

Tissues, cell lines, primary or sorted cells etc. yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Blood yes_icon yes_icon yes_icon yes_icon yes_icon* yes_icon* yes_icon yes_icon yes_icon
Liquid biopsy (CSF, serum, plasma, urine) yes_icon no yes_icon no yes_icon M yes_icon yes_icon yes_icon* no
Exosomes yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon* no
Illumina yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Ion Torrent no no no yes_icon yes_icon no no no yes_icon
Oxford Nanopore no no no no no no no no yes_icon M
Pacific Biosciences no no no no no no no no yes_icon M
Single-read yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
Paired-end yes_icon yes_icon yes_icon yes_icon no yes_icon yes_icon no yes_icon
Dual indexing compatible yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no
Automation-Compatible yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no no
UMIs available included included included no yes_icon no yes_icon no yes_icon
Data Analysis pipeline available yes_icon yes_icon yes_icon yes_icon included included yes_icon yes_icon yes_icon
Service available yes_icon yes_icon yes_icon no yes_icon yes_icon yes_icon yes_icon no
Spike In control recommendation SIRV-Set 1/2/3 SIRV-Set 3 no no SIRV-Set 1/2/3 yes_icon
8 rxn kit no no no yes_icon no no no yes_icon yes_icon
24 rxn kit yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon
96 rxn kit yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon yes_icon no
2×96 rxn kit no no no no yes_icon yes_icon no no no
384 rxn kit no no no no yes_icon no no no no
Trial kit available (4 preps) yes_icon no yes_icon yes_icon yes_icon yes_icon no no yes_icon

Legend:

yes_icon* Optimal Choice
yes_icon Yes
yes_icon Possible, but not evaluated. For more information please contact support@lexogen.com.
yes_icon pA Possible only if a poly(A) tail (and a 5′ cap for TeloPrime) is present or with prior poly-adenylation
yes_icon M Protocol modifications apply
no Not possible or not useful