SPLIT RNA Extraction Kit
The SPLIT RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into two fractions, large RNA and small RNA, facilitating the analysis of e.g., mRNA and miRNA from the same sample. Thus the RNA obtained is ideal for seamlessly preparing libraries for Next Generation Sequencing of total RNA or its large and small fractions or any other demanding downstream application. The new SPLIT RNA Extraction Kit for Blood enables concomitant depletion of globin mRNAs from human blood samples in low volumes (50 – 250 µl).
The SPLIT RNA Extraction Kit contains reagents for the isolation of total RNA or the large RNA fraction from 48 samples, or small and large RNA fractions from 24 samples.
chaotropic to facilitate effortless and complete solubilization.
The SPLIT for Blood protocol adds a red blood cell lysis step
prior to sample homogenization.
a special gel matrix that acts as a barrier between the organic and
aqueous phase based on the density differences.
solution, which is essential for the efficient separation of genomic
DNA into the organic phase. Chloroform is added and phases are
and no carry-over of the organic phase will take place.
(1.75x isopropanol) can be extracted or the RNA can be split in a large
(0.33x isopropanol) and a small RNA fraction (1x isopropanol to the
flow-through of the large RNA fraction).
the entire RNA will precipitate onto the silica carrier, while for the
large fraction RNA with a lower limit of about 150 nt will bind
whereas the small RNA will be in the flow-through.
which didn´t bind to the silica column. After addition of 1x isopropanol
it can be precipitated and purified onto a new silica column.
membrane to elute the RNA bound to the silica carrier.
For viewing the whole workflow on page please click here
Frequently Asked Questions
Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact firstname.lastname@example.org.
The SPLIT RNA Extraction Kit for Blood protocol is verified for use with 50 – 250 µl of fresh human blood collected in EDTA containing blood collection tubes. Please contact email@example.com if you wish to use this protocol with blood from other species, variable input blood volumes, or with blood exposed to other collection methods or storage conditions.
The protocol is verified for use with 50 – 250 µl of fresh human blood collected in EDTA containing blood collection tubes. Please contact firstname.lastname@example.org if you wish to use this protocol with blood from other species, variable input blood volumes, or with blood exposed to other collection methods or storage conditions.
|Problem||Likely cause||Comments and suggestions|
|No or poor phase separation||Cooled phase-lock gel or insufficient centrifugation||
|Incorrect cut-off||Volume of isopropanol addition||
|Degraded RNA||RNA source||
|Low adsorption ratios (i.e., peak at 230 nm)||Contamination with organic solvents, salts, or metal ions||
|gDNA contamination||Contamination with organic phase||
|Low or no RNA yield||RNA remains on spin column||
|Spin column was overloaded||
|Problem in downstream application||Salt or ethanol carry-over during elution||
SPLIT RNA Extraction Kit
User Guide – update 05.08.2019
Application Note – SPLIT RNA Extraction Kit
Product Flyer – SPLIT RNA Extraction Kit for Blood
SPLIT Supplementary Protocol: Purification of RNA from FFPE Samples – upload 18.07.2018
Material Safety Datasheets
SPLIT RNA Extraction Free Trial Kit
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