The SPLIT RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into two fractions, large RNA (> 150 nt) and small RNA (< 150 nt), facilitating the analysis of e.g. mRNA and miRNA from the same sample. Thus the RNA obtained is ideal for seamlessly preparing libraries for Next Generation Sequencing of total RNA or its large and small fractions.
With the content of the kit total RNA or the large RNA fraction can be extracted from 48 samples. If the small RNA fraction should be purified in addition to the large RNA fraction, then 24 samples can be extracted.
The SPLIT RNA Extraction Kit for Blood additionally includes the Blood Lysis Buffer for lysis of red blood cells prior to sample homogenization of the conventional SPLIT RNA Extraction Kit. This additional step enables efficient depletion of globin mRNA from the resulting RNA sample from as little as 50 to 250 µl of human blood.
- Buffers and Purification Columns
- Phase-Lock Gel Columns