Ultra-low Input RNA Sequencing
Not sure if you have enough RNA for RNA-Seq to work?
Send your samples our way!
Ultra-low Input RNA Sequencing
Ultra-low input RNA-Seq enables insight into gene expression profiles even when dealing with very low inputs. This is often the case when working with rare cell types, having access to a limited amount of material, or being interested in lowly abundant transcripts present only in a small population of cells. We have substantial experience making low and ultra-low input RNA-Seq work and providing accurate and reliable transcriptomic datasets from these limited samples. Our proprietary, high-definition technology enables us to work with RNA input as low as 10 pg and achieve unprecedented sensitivity and reproducibility, even with low-quality samples.
Benefits
- Ultra-low input: 10 pg to 1 ng of total RNA*, corresponding to 1-100 cells.
- Supreme sensitivity results in more detected genes than other high-throughput technologies.
- Detection of lowly expressed transcripts (<10 copies/cell) possible
- No more gDNA in your reads, thanks to our proprietary technology
- We also accept low-quality RNA
*Our technology enables us to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts.
Applications
- High-definition single-cell differential gene expression analysis
- Post-bulk scRNA-Seq characterization of cell subpopulations
- Gene expression analysis of rare cell types or cells that are scarce, such as circulating tumor cells (CTCs) or innate lymphoid cells (ILCs).
- Subcellular (cytoplasm, organelle) RNA analysis
- Single nucleus RNA-Seq
- Gene expression analysis of low abundant transcript analysis
Deliverables
- Raw data
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts + counting summary (.tsv)
- Differential expression + DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us lysates (e.g., from FACS-sorted cells) or purified RNA in ultra-low input range (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are expected. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (see Lexogen Bioinformatics Services page). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
- Shipping ultra-low input RNA samples requires our RNA expert input.
Sample Preparation
- Sorted and lysed cells, or purified, ultra-low input RNA as starting material.
- Low-quality and challenging samples, e.g., FFPE material, possible.
- Sample QC included (where possible).
Library Preparation
- Library QC included.
- Using proprietary Lexogen technologies, adapted to your needs.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
