Single-cell RNA Sequencing
Unravel cellular diversity by diving into the transcriptome at the single-cell level.
Single-cell RNA Sequencing
Single‐cell RNA sequencing (scRNA‐seq) has become the state‐of‐the‐art approach for studying the heterogeneity and complexity of transcripts within individual cells. scRNA-Seq allows us to characterize and distinguish cells at the transcriptome level, enabling us to identify rare but functionally significant cell populations in health and disease and tackle experimental questions that cannot be answered using the bulk analysis approach.
Benefits
- State-of-the-art, high-throughput scRNA-Seq
- Processing various tissue types and cell suspensions
- Pilot experiments for proper optimization offered
- In-depth data analysis available
Applications
- Reveal the uniqueness of the individual cells to explore novel targets and biomarkers
- Explore cell heterogeneity
- Study differentiation and development on a cellular level
- Follow disease and treatment progress on a cellular level
- Explore cellular networks
- Discover novel cell populations
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Cell ranger basic analysis: annotation with cellranger mkref, cell ranger count, clustering, differential gene expression, PCA, T-SNE, UMAP embeddings
- more advanced analysis or outputs is available, e.g., Doublet detection, processing with Seurat pipeline, heatmaps, trajectory inference etc.
Working with us
Samples for single-cell RNA sequencing should be collected and prepared carefully to maximize cell viability and nuclear integrity. We assists you along this process and customize our workflow to your needs. Our Lexogen Bioinformatics Services can provide further analyses or outputs for your single-cell RNA-Seq study.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Preparation
- We accept frozen tissue, as well as cryopreserved and fresh single-cell suspension samples.
- For other sample types, please consult with us via services@lexogen.com
- Sample QC includes Cell Viability QC and/or Nuclei QC.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC is included.
Sequencing
- Read depth most suitable for your single-cell RNA setup.
Data Analysis and Report
- Data are analyzed in-house after performing data quality control.
- Basic, extended, and more advanced data analysis packages are available.
