Gene Expression Profiling
Gene Expression Profiling service provides a cost-efficient genome-wide analysis of gene expression using Lexogen’s proprietary QuantSeq technology, targeting 3’ ends of mRNA molecules. QuantSeq technology offers a perfect solution for identifying and comparing gene expression levels between experimental groups, be it treatment, different genotypes, or disease-related research. As input material, we use total RNA. No prior poly(A) enrichment or rRNA depletion is needed.
Benefits
- Cost-efficient gene expression analysis
- Focus on polyadenylated transcripts
- Works with low-input material
- Compatible with low-quality RNA
Applications
- Cost-efficient Differential gene expression
- High-throughput gene expression sequencing, e.g., for compound screening*
- Drug discovery & development studies, e.g., with High-throughput kinetic RNA-Seq (after SLAMseq)
- FFPE Transcriptomics
- Whole Blood Transcriptomics
- Plant Transcriptomics
- Targeted RNA sequencing*
- Determination of alternative poly(A) sites
*If you would like to do targeted RNA sequencing with us, please contact our team at services@lexogen.com to discuss your requirements.
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts and counting summary (.tsv)
- Differential expression and DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are expected. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
