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RNA kinetics
RNA kinetics is the analysis of RNA metabolism capturing all biochemical processes along the life cycle of ribonucleic acid (RNA) molecules from synthesis to degradation, including events such as regulation, processing, and modification. Metabolic RNA labeling is used to assess RNA kinetics specifically by detecting anabolic (synthesis) and catabolic (degradation) processes of RNA over time and in response to external stimuli, such as treatments. High-throughput kinetic RNA sequencing describes the global analysis of RNA kinetics by RNA sequencing to discover de novo synthesis and degradation of all affected RNA molecules in an unbiased manner.
SLAMseq Applications
Kinetic or metabolic RNA sequencing (RNA-Seq) combines labeling of nascent RNA transcripts with RNA-Seq readout to study RNA biosynthesis and turnover in a time-resolved manner. SLAMseq is commonly used to:
- perform gene regulation studies,
- uncover cell signaling pathways,
- assess mRNA half-life and transcript stability,
- reveal effects and synergies of (fast-acting) drugs,
- identify and validate drug targets or biomarkers,
- differentiate between primary and secondary targets,
- and test for viral contaminants in pharmaceutical production.
How does SLAMseq work?
SLAMseq (Thiol (SH)-Linked Alkylation for the Metabolic sequencing of RNA) enables the identification and quantification of newly synthesized (nascent) and existing RNA in living cells, without the need for lengthy biochemical enrichment procedures.
Watch a short video to learn more about SLAMseq:
Benefits
SLAMseq unites the patented 4-thiouridine (S4U) labeling technology with a streamlined workflow in the only commercially available, high-quality kit and is the method of choice to determine treatment effects on RNA synthesis or RNA turnover in cell culture experiments.
Lexogen holds the exclusive license for the SLAMseq technology and offers complete end-to-end solutions, including:
- explorer modules,
- kinetics kits for Metabolic RNA Labeling,
- various compatible library prep kits,
- and data analysis options.
RNA from SLAMseq experiments can also be conveniently processed at Lexogen NGS Services for library prep, sequencing, and data analysis.
Nascent RNA labeling
Time-resolved RNA analysis
Licensed technology
End-to-end solution
Please get in contact with our Partnering Business or reach out to partnering@lexogen.com, if you are interesting in adopting this licensed technology in your facility.
Not sure how to get started with your SLAMseq experiment?
Try the SLAMseq product configurator and find the ideal setup for your application!
