Whole Transcriptome Sequencing
We empower your research: get insight into all of your RNAs!
Whole Transcriptome Sequencing
Lexogen’s NGS Whole Transcriptome Sequencing Service is perfect if you are interested in getting insight into ALL transcripts present in your samples, both coding and noncoding, including lncRNA, snRNA, snoRNA, circRNA, etc. We utilize our proprietary technology to enable insight into all of the RNAs in your sample.
We offer both Whole Transcriptome mRNA Sequencing Service and Whole Transcriptome Total RNA Sequencing Service.
Our Whole Transcriptome Sequencing offer
Whole Transcriptome mRNA-Seq
Whole Transcriptome mRNA-Seq
If you would like to look at gene expression only, we recommend our Gene Expression Sequencing Service. However, if you want to get the deepest insight into mature, polyadenylated RNAs and identify isoforms and alternative splicing, among other things, the Whole Transcriptome mRNA Sequencing Service is a perfect choice.
Our Whole Transcriptome mRNA Sequencing workflow includes a poly(A) selection step.
Benefits
- Full focus on the coding, mature polyadenylated transcripts
- Ideal for low-input materials
- UMIs included
Applications
- Gene and transcript expression analysis
- Isoform identification
- Alternative splicing patterns identification, including exon-intron boundaries and gene fusions
- SNP detection
- Whole blood mRNA-Seq
Whole Transcriptome Total RNA-Seq
Whole Transcriptome Total RNA-Seq
The total RNA-Seq approach offers the most comprehensive analysis of the transcriptome. It is an ideal choice if you deal with RNA degradation, e.g., after FFPE fixation and laser microdissections. We know how precious these samples are and how challenging obtaining transcriptome profiles from them may be. This is why our RNA experts have developed workflows allowing us to process, prepare, and analyze these challenging samples, including a step of ribosomal RNA (rRNA) depletion, to allow more sequencing reads to be focused on desired transcripts, especially if you are interested in lowly abundant transcripts.
Benefits
- Compatible with low-input material
- Ideal for low-quality RNA
- UMIs included
- rRNA depletion for various species available
Applications
- Analysis of coding and various types of noncoding RNAs, including lncRNA, snRNA, snoRNA, circRNA, etc.
- Transcriptome assembly
- Isoform identification
- Biomarker identification
- SNP detection
- FFPE Transcriptomics
- Whole Blood Transcriptomics
- Plant Transcriptomics
- Bacteria Transcriptomics
Deliverables
- Demultiplexed raw files (FASTQ)
- Trimmed files (FASTQ)
- UMI extracted files (FASTQ)
- Aligned files (BAM)
- UMI collapsed files (BAM)
- RSeQC (.txt)
- Gene counts and counting summary (.tsv)
- Isoform quantification (.dat)
- Differential expression and DE summary (.tsv)
- MultiQC reports – raw, trimmed, extracted, alignment, RSeQC (.html)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
