DNA Sequencing
Next-generation sequencing (NGS) technologies enable researchers to investigate the genetic basis of biological function, variation, and disease with increasing depth and throughput. Various DNA sequencing approaches are available to facilitate numerous applications, including variant discovery, genome assembly, resequencing, phylogenetics, genotyping, and taxon identification.
DNA sequencing services from Lexogen can start with DNA extraction or library preparation, with optional target enrichment. We use top-quality library generation methods with customized sequencing and data analysis approaches according to individual project requirements and target applications. DNA extraction can be performed upon request, e.g., from cell pellets, fresh or flash-frozen tissues, and FFPE curls*.
We also offer various RNA sequencing options if you are also interested in studying the transcriptome. Learn more here.
* For other sample types not listed, please enquire at services@lexogen.com
Benefits
- High-quality data
- Consultations with PhD-level NGS experts
- Non-standard applications, full flexibility
- State-of-the-art sequencing platforms by Illumina and Element Bioscience (AVITI)
Applications
- Whole-genome sequencing
- Exome sequencing
- Target enrichment
- Amplicon sequencing
Deliverables
- Raw FASTQ files
- FastQC/MultiQC reports
- Trimmed FASTQ files (upon request)
- .BAM files (reference genome alignments)
- .BW coverage files (.e.g. for IGV/other genome browser uploads)
- Alignment summary metrics (mismatches, indels computed with Picard tools)
- Target regions coverage as text files and figures
Deliverables are summarized in a PDF report.
Working with us
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- DNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
