Small RNA Sequencing
Small RNAs (sRNAs) are less than 200 nt long, usually non-coding RNA molecules, that have key functional roles in mRNA turnover, translational regulation, and chromatin compaction, among others, and are important regulators of gene expression. MicroRNAs (miRNAs) are the most well-known and studied class of small RNAs, which modulate protein expression through post-transcriptional regulation. Abnormal mechanisms of miRNAs are reported in various diseases, especially cancer. Nowadays, many miRNAs serve as biomarkers of cancer.
Lexogen’s Small RNA Sequencing service allows you to get a complete small RNA profile across the entire transcriptome, from miRNAs and siRNAs to piRNAs, snoRNAs, and others.
Benefits
- Wide-input range
- Start from total RNA or enriched sRNAs (e.g., RISC-associated sRNAs)
- Optimized for low-input samples (e.g., liquid biopsies and extracellular vesicles)
- Extraction from challenging material or exotic species
Applications
- Expression profile of small RNAs, including miRNAs
- Identification of new small RNAs
- miRNA isoforms (isomiRs)
- Post-transcriptional regulation by small RNAs on phenotypes
- Discovery of novel biomarkers for cancer and other diseases
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Small RNA miRTrace QC (.html)
- mature miRNAs and hairpin miRNAs counts and counting summary (.tsv)
- Differential expression and DE summary of mature miRNAs and hairpin miRNAs (.tsv)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are expected. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
