Lane Mix Calculation
Libraries should ideally be pooled in an equimolar ratio for multiplexed sequencing. It is important to ensure accurate quantification of the individual libraries prior to pooling, as well as for the library pool (lane mix). To quantify your libraries measure the concentration using either qPCR or fluorescence-based assays and determine the average library size, using microcapillary electrophoresis analysis (e.g., Bioanalyzer or Fragment Analyzer). The Library Quantification File will calculate the molarities of each library from the concentration measurement and average size. Further it will provide the volumes of each library to be used for preparation of an equimolar lane mix.