Delivery should be in 1.5 mL tubes, avoiding error-prone plates and tube-strips. We kindly ask to send RNA samples on dry ice. Readily prepared libraries should be delivered in a cooled parcel. Shipment over weekends should be avoided to prevent samples from staying at ambient temperatures for prolonged periods of time.
Please see the sample submission form and submission recommendations for more information.
Raw sequencing files and results from data analysis will be provided for convenient and secure download from Lexogen’s sftp servers in Vienna, Austria. Other options for data transfer are available upon request.
Services covers applications like gene expression profiling, mRNA sequencing, whole transcriptome analysis (including non-polyadenylated RNAs and non-coding RNAs), small RNA sequencing, single cell RNA-Seq and ultra-low input RNA sequencing.
All RNA-Seq library preparation methods offered by Lexogen in kit format can be ordered as part of our Services. This includes QuantSeq FWD, REV, and Flex, CORALL Total RNA-Seq, CORALL mRNA-Seq, Small RNA-Seq library prep, and LUTHOR.
Please inquire for not listed applications, methods, and services.
Yes. Remaining material may be returned to you. Please make sure to inform the Services team before the project begins.
Most of our protocols are compatible with low input and degraded samples, including material from FFPE samples. RNA will be characterized prior to library preparation to ensure appropriate workflow parameters.
Due to the wide range of different Services projects a strict maximum processing time cannot be promised. For standard setups, please expect data availability within four to six weeks from receipt of your samples.
We appreciate any information regarding quantity and quality of your samples. However, the quality control (QC) via UV-Vis spectrophotometry (NanoDrop) and capillary electrophoresis (BioAnalyzer/ Fragment Analyzer) is included in the Services bundle, and these measurements do not need to be performed prior to sample submission.
In such case, we will contact you with the results of our internal quality control. Upon your confirmation, we will omit the specified sample(s) from downstream processing. The amount in the final invoice will be reduced accordingly to account for the excluded sample(s).
We do accept sequencing-only projects, starting from prepared lane mixes. These projects need to be well coordinated with careful selection of the indices. Please utilize the ‘Lane Mix Calculation’ file located in the download section of the Lexogen Services webpage as a tool to quantify your libraries prior to pooling. For more information, please contact firstname.lastname@example.org.
Data analysis includes basic statistics (number of input reads, number of uniquely mapping reads, % of uniquely mapping reads, average input read length), alignment rates, gene counts, read distribution statistics, information on sample reproducibility, information on differential expression analysis and visualization.
Unless specified otherwise, the libraries are sequenced in single read (SR) mode using the high output 75 cycle cartridge. Sequencing depth depends on library type and project requirements and will be discussed prior to sample submission.