Your samples and Lexogen’s expertise

Lexogen develops world-class RNA-Seq technologies, and Services applies this knowledge to your samples by providing a fully integrated workflow from samples to reporting. Sequence and profile known and unknown RNAs in any species, quantify transcript expression accurately, and detect novel and rare transcripts as well as single nucleotide polymorphisms (SNPs). You can contact us via the inquiry form, or write to us at services@lexogen.com.

Applications

Cost-efficient genome wide analysis of gene expression using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD

Advantages:

  • Only one fragment per transcript sequenced
  • Few reads required (2-5 M / sample)
  • Individual library prep for each sample
  • UMIs and globin mRNA blocker available

Input requirements:

  • Intact and degraded samples such as FFPE-derived RNAs
  • Samples of varying RNA integrity and mRNA content
  • Suitable for SLAMseq RNA samples

Targeted transcripts:

  • Polyadenylated RNAs
Gene expression profiling for large screening projects using the QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit

Advantages:

  • Highest cost efficiency by early sample barcoding and pooling
  • Only one fragment per transcript sequenced
  • Few reads required (2-5 M / sample)
  • Easy scaling from 8 to 36,864 samples

Input requirements:

  • Samples homogeneous in RNA amount, mRNA content and RNA integrity

Targeted transcripts:

  • Polyadenylated RNAs
Sequencing of polyadenylated RNAs using the CORALL mRNA-Seq Library Prep Kit

Advantages:

  • Full sequence coverage of mature, polyadenylated RNAs (mRNAs)
  • Isoform detection
  • SNP detection

Input requirements:

  • RNAs with high integrity

Targeted transcripts:

  • Polyadenylated RNAs
Universal RNA sequencing using the CORALL Total RNA-Seq Library Prep Kit and the RiboCop rRNA Depletion Kits

Advantages:

  • Full sequence coverage of total RNA
  • Isoform detection
  • SNP detection

Input requirements:

  • Intact and degraded samples such as FFPE-derived RNAs

Targeted transcripts:

  • Polyadenylated and non-polyadenylated RNAs
Sequencing and profiling of small RNAs using the Small RNA-Seq Library Prep Kit

Advantages:

  • Sequencing of miRNAs, piRNAs, siRNAs, etc
  • Wide input range from 50 pg to 1,000 ng RNA
  • High reproducibility and sensitivity

Input requirements:

  • Total RNA and enriched small RNA input
  • Suitable for low concentrated RNA (e.g. from plasma, serum, and urine)

Targeted transcripts:

  • RNAs shorter than 400 nt with 5′ P and 3′ OH ends
High resolution gene expression profiling of single cells and ultra-low input RNA samples using the LUTHOR 3’ mRNA-Seq Library Prep Kit

Advantages:

  • Unprecedented sensitivity and reproducibility for individual cells
  • Novel direct RNA amplification technology combined with an efficient one-step 3’ RNA-Seq library preparation method

Input requirements:

  • Animal cells, protoplasts
  • Purified RNA from all organisms and viruses

Targeted transcripts:

  • Polyadenylated RNAs

For a comprehensive overview of applications, additional options, and RNA input and sample types, please
pdf Download the RNA-Seq Library Prep Document Overview

Further, we can

  • prepare libraries from SLAMseq samples (metabolic labeling).

  • use Lexogen’s 12-nt UDI barcodes to provide superior error correction for maximal data output.

  • include SIRVs and ERCC spike-in control transcripts for workflow validation and data set concordance.

  • deplete and block globin mRNA in blood samples for significantly improved sensitivity.

  • include Unique Molecular Identifiers (UMIs) to take care of PCR duplication in low-complexity samples.

  • handle large-scale projects on automated liquid handling platforms.

  • flexibly adjust to your requirements for advanced setups and workflows.

Inquire for Services

Benefit from 5% discount on Services until April 30th, 2021 by using this form. See blog article for details and Terms & Conditions.

Which RNA-Seq Services are you interested in? *
3’ mRNA-Seq (gene expression profiling)mRNA-Seq (whole transcript analysis with poly(A) selection)Total RNA-Seq (whole transcriptome analysis with ribo-depletion)Single-cell RNA-SeqSmall RNA-SeqSLAMseq Metabolic LabelingOther:

Do you need RNA extraction service? *
YesNo

Do you need bioinformatics analysis service? *
YesNo

Species and type of cells/tissue

Number of samples

Description of your project

Additional comments/questions

How did you find about Lexogen Services? *
Recommendation from colleague or friendFrom Sales Rep or Lexogen DistributorBy searching the InternetOther:

Type the characters:
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FAQ

Delivery should be in 1.5 mL tubes, avoiding error-prone plates and tube-strips. We kindly ask to send RNA samples on dry ice. Readily prepared libraries should be delivered in a cooled parcel. Shipment over weekends should be avoided to prevent samples from staying at ambient temperatures for prolonged periods of time.
Please see the sample submission form and submission recommendations for more information.

Raw sequencing files and results from data analysis will be provided for convenient and secure download from Lexogen’s sftp servers in Vienna, Austria. Other options for data transfer are available upon request.

All RNA-Seq library preparation methods offered by Lexogen in kit format can be ordered as part of our Services. This includes QuantSeq FWD, REV, and Flex, CORALL Total RNA-Seq, CORALL mRNA-Seq, Small RNA-Seq library prep, and LUTHOR. Further, we offer RNA extraction, preparation of RNA-Seq libraries from SLAMSeq samples, and comprehensive data analysis.

Lexogen is open to provide Services for customers from any country. Samples will be processed at Lexogen’s headquarters in Vienna, Austria.

Samples from most organisms are accepted. Please note that compatibility depends on workflow selection. For more information regarding your species of interest please contact services@lexogen.com.

Yes. Remaining material may be returned to you. Please make sure to inform the Services team before the project begins.

Lexogen RNA-seq library preparation kits are compatible with low-input samples. For exact measures please contact services@lexogen.com.

Most of our protocols are compatible with low input and degraded samples, including material from FFPE samples. RNA will be characterized prior to library preparation to ensure appropriate workflow parameters.

Due to the wide range of different Services projects a strict maximum processing time cannot be promised. For standard setups, please expect data availability within four to six weeks from receipt of your samples.