Additional Modules for SENSE Kit:

dualbarcodemodule_550
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i5 Dual Indexing Add-on Kit for QuantSeq/SENSE

The i5 Dual Indexing Add-on Kit contains four perfectly balanced i5 Indices 5001-5004 (Cat. No. 047).

i5 (Index 2) indices can be introduced at the PCR step of SENSE mRNA-Seq V2 for Illumina (Cat. No. 001), SENSE Total RNA-Seq for Illumina (Cat. No. 009), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the four i5 indices can be combined with any of the 96 i7 indices (Index 1) (BC01-96) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 384 different barcoding options.

  • Kit size: 24 rxn or 96 rxn of each i5 index
  • Compatibility information
    • Product: SENSE mRNA-Seq V2, SENSE Total RNA-Seq, and QuantSeq
    • Platform: Illumina

pdf i5 Dual Indexing Add-on Kit for QuantSeq/Sense Instruction Manual

PCRaddonkitillumina_550
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PCR Add-on Kit for Illumina

The PCR Add-on kit contains a PCR Mix including an Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode referred to as Barcode 00 for 96 PCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay to determine the optimal number of cycles for the endpoint PCR of your QuantSeq or SENSE cDNA libraries can be determined.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values.

The kit furthermore contains a reamplification primer that can be used to reamplify already barcoded libraries if they were undercycled to get enough material for sequencing.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: SENSE mRNA-Seq V2, SENSE Total RNA-Seq, and QuantSeq
    • Platform: Illumina

pdf PCR Add-on Kit for Illumina Instruction Manual

sense barcode kit
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Barcodes for SENSE mRNA-Seq Library Prep Kit

96 external barcodes for the SOLiD and 24 in-line barcodes for the Ion Torrent system are available for the SENSE mRNA-Seq library prep kit. Each Barcode Set contains 12 different barcodes with 8 or 24 rxn.

  • Kit size: 8 or 24 rxn
  • Compatibility information
    • Product: SENSE mRNA-Seq
    • Platform: Ion Torrent or SOLiD
Automationmodulesense
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Automation Module for SENSE mRNA-Seq V2

The Automation Module for SENSE mRNA-Seq is part of the autoSENSE package. Together with the SENSE mRNA-Seq V2 kit and the autoSENSE software it provides a fully automated walk-away mRNA-Seq library preparation protocol and the possibility to select for larger library sizes.

  • Kit size: 96 preps
  • Compatibility information
    • Product: SENSE mRNA-Seq V2
    • Platform: Illumina
Purificationmodule
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Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: SENSE all products / QuantSeq all products / TeloPrime
    • Platform: Illumina / Ion Torrent / SOLiD
BarcodeSenseQuantSeq_550
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i7 Index Plate for QuantSeq/SENSE for Illumina

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or SENSE kit for Illumina.

NOTE: By 17.02.2017 barcodes have been renamed (i7 Index Plate (7001-7096), formerly Barcode Plate (BC01-96)) and rearranged for a better nucleotide balance for sequencing when only few samples are multiplexed. Moreover, all indices are now different from Illumina barcodes (former BC05 – TAATCG) has been removed.

pdf  Lexogen for Illumina Index Primer Overview

Additional Modules for QuantSeq Kit:

dualbarcodemodule_550
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i5 Dual Indexing Add-on Kit for QuantSeq/SENSE

The i5 Dual Indexing Add-on Kit contains four perfectly balanced i5 Indices 5001-5004 (Cat. No. 047).

i5 (Index 2) indices can be introduced at the PCR step of SENSE mRNA-Seq V2 for Illumina (Cat. No. 001), SENSE Total RNA-Seq for Illumina (Cat. No. 009), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the four i5 indices can be combined with any of the 96 i7 indices (Index 1) (BC01-96) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 384 different barcoding options.

  • Kit size: 24 rxn or 96 rxn of each i5 index
  • Compatibility information
    • Product: SENSE mRNA-Seq V2, SENSE Total RNA-Seq, and QuantSeq
    • Platform: Illumina

pdf i5 Dual Indexing Add-on Kit for QuantSeq/Sense Instruction Manual

PCRaddonkitiontorrent
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PCR Add-on Kit for Ion Torrent

The PCR Add-on kit contains a PCR Mix including Ion Torrent specific primer and a thermostable polymerase. With the PCR Add-on kit more endpoint PCRs can be run for your sample to determine the exact number of cycles needed to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values. If your sample is undercycled the kit can furthermore be used to add more cycles and reamplify your library.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq
    • Platform: Ion Torrent
QSFlexfirststrand
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QuantSeq-Flex First Strand Synthesis Module

The QuantSeq-Flex First Strand Synthesis Module enables the exchange of the reverse transcription primer with a target specific primer in the QuantSeq protocol. It can furthermore be used to generate longer libraries with the standard QuantSeq 3’mRNA-Seq kit if needed.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq FWD
    • Platform: Illumina
QSFlexsecondstrand
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QuantSeq-Flex Second Strand Synthesis Module V2

With the QuantSeq-Flex Second Strand Synthesis Module V2 the random second strand synthesis primer can be substituted with a target-specific primer in the QuantSeq protocol. Please note that the second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need lower temperatures, please contact info@lexogen.com.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq FWD
    • Platform: Illumina
PCRaddonkitillumina_550
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PCR Add-on Kit for Illumina

The PCR Add-on kit contains a PCR Mix including an Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode referred to as Barcode 00 for 96 PCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay to determine the optimal number of cycles for the endpoint PCR of your QuantSeq or SENSE cDNA libraries can be determined.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values.

The kit furthermore contains a reamplification primer that can be used to reamplify already barcoded libraries if they were undercycled to get enough material for sequencing.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: SENSE mRNA-Seq V2, SENSE Total RNA-Seq, and QuantSeq
    • Platform: Illumina

pdf PCR Add-on Kit for Illumina Instruction Manual

BarcodeSenseQuantSeq_550
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i7 Index Plate for QuantSeq/SENSE for Illumina

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or SENSE kit for Illumina.

NOTE: By 17.02.2017 barcodes have been renamed (i7 Index Plate (7001-7096), formerly Barcode Plate (BC01-96)) and rearranged for a better nucleotide balance for sequencing when only few samples are multiplexed. Moreover, all indices are now different from Illumina barcodes (former BC05 – TAATCG) has been removed.

pdf  Lexogen for Illumina Index Primer Overview

Additional Modules for TeloPrime Kit:

pcraddonkitteloprime
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TeloPrime PCR Add-on Kit

The TeloPrime PCR Add-on Kit contains 30 reactions of the PCR Mix, the polymerase and the Forward and Reverse Primer separately. Based on this primers can be easily exchanged with gene-specific primers. The kit can furthermore be used to run multiple PCR reactions of your sample to generate sufficient template for any downstream application.

  • Kit size: 30 rxn
  • Compatibility information
    • Product: TeloPrime