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Modules and Add-ons

Additional Modules for QuantSeq Kits:

PCR Add-on and Reamplification Kit V2 for Illumina (Cat. No. 208)

The PCR Add-on and Reamplification Kit V2 contains a PCR Mix, a thermostable polymerase, Illumina P5 and P7 primers without barcodes for 96 qPCR reactions. By adding SYBR Green I to the PCR reaction, a qPCR assay can be set up to determine the optimal number of cycles for the endpoint PCR of your QuantSeq V2, QuantSeq-Pool, or CORALL V2 cDNA. ATTENTION: use of this kit is not recommended for qPCR assays with previous versions of QuantSeq or CORALL.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too low library yield, while overcycling can lead to distortions in gene expression values.

In addition, the kit contains a reamplification primer mix (RE) that can be used instead of P5 and P7 primers to reamplify barcoded final libraries to increase the yield for sequencing or after use of enrichment panels. This kit is recommended for library reamplification for all Lexogen library prep kits.

  • Kit size: 96 qPCR rxns or 96 re-amplification rxns.
  • Compatibility information:
    ATTENTION: The qPCR assay is recommended for use with QuantSeq with UDI V2, QuantSeq-Pool, and CORALL RNA-Seq V2. Do not use this kit for qPCR assays with previous versions of QuantSeq or CORALL!
    Library reamplification is recommended for all Lexogen library prep kits.
  • Product
    • qPCR:
      • CORALL RNA-Seq V2 with UDI (Cat. No. 171 – 186),
      • QuantSeq V2 with UDI (Cat. No. 191 – 196),
      • QuantSeq-Pool (Cat. No. 139).
    • Library reamplification:
      • All CORALL library prep kits (all versions),
      • All QuantSeq library prep kits (all versions),
      • QuantSeq-Pool,
      • LUTHOR library prep, and
      • Small RNA-Seq Library prep.
    • Platform: Illumina

Download the User Guide from the Documents section.

UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1)

The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Cat. No. 081.96) contains the UMI Second Strand Synthesis Mix (USS). This mix simply replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq FWD Kit. The module allows unique tagging of individual transcripts with 6 nt long Unique Molecular Identifiers (UMI) located between the partial P5 adapter and the random priming sequence. Automated data analysis for QuantSeq FWD-UMI libraries is available. For more information, please see www.lexogen.com/data-analysis-solutions.

Use this module to identify PCR duplicates and eliminate amplification bias.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3‘ mRNA-Seq FWD
    • Platform: Illumina

Globin Block Modules for QuantSeq

The Globin Block (RS-Globin Block) Modules for QuantSeq prevent the generation of library fragments from globin mRNAs, by blocking their extension during second strand synthesis. The module is compatible only with QuantSeq 3’ mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, and REV, Cat. No. 016), and is intended for the preparation of libraries from blood RNA samples.

Each module provides a modified RNA Removal Solution (RS-Globin Block) containing species-specific globin blocking oligos. The RS-Globin Block solution replaces the RNA Removal Solution (RS) from the standard QuantSeq 3’ mRNA-Seq Library Prep Kits. Separate modules are available for human (Homo sapiens) and pig (Sus scrofa).

The RS-Globin Block, Homo sapiens module (RS-GBHs, Cat. No. 070.96) is designed for human blood RNA and contains blocking oligos designed against the human alpha and beta hemoglobin chain mRNAs: HBA1HBA2, and HBB.

The RS-Globin Block, Sus scrofa module (RS-GBSs, Cat. No. 071.96) is designed for pig blood RNA and contains blocking oligos designed against the pig alpha and beta hemoglobin chain mRNAs: HBA and HBB.

NOTE: The Globin Block Modules are also compatible with QuantSeq Kits released prior to the kit upgrade on 7th February, 2017. Please see the QuantSeq FAQs for details.

  • Kit Size: 96 rxn
  • Compatibility information:
    • Product: QuantSeq 3′ mRNA (FWD and REV)
    • Platform: Illumina

BC1 Block Module for QuantSeq

The BC1 Block (RS-BC1 Block) Module for QuantSeq prevents the generation of library fragments from the abundant BC1 transcripts that are present in mouse brain samples (Mus musculus, Mm), by blocking their extension during second strand synthesis. The module is compatible with QuantSeq 3’ mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, 113 – 115, 129 – 131, and REV, Cat. No. 016), and is intended for the preparation of libraries from mouse brain samples.

The module provides a modified RNA Removal Solution (RS-BC1 Block) containing transcript-specific BC1 blocking oligos. The RS-BC1 Block solution replaces the RNA Removal Solution (RS) from the standard QuantSeq 3’ mRNA-Seq Library Prep Kits.

  • Kit Size: 96 rxn
  • Compatibility information:
    • Product: QuantSeq 3′ mRNA (FWD and REV)
    • Platform: Illumina

QuantSeq-Flex First Strand Synthesis Module V2

The QuantSeq-Flex Targeted RNA-Seq Modules complement the QuantSeq FWD kits and enable the generation of targeted RNA-Seq libraries from any RNA sample using custom primers for first or second strand synthesis.

With the QuantSeq-Flex First Strand Synthesis Module V2 the reverse transcription primer of QuantSeq FWD can be substituted with custom target-specific primers.

The new QuantSeq-Flex First Strand Synthesis Module V2 can further be used to modulate the insert size or to enable larger RNA volumes for standard QuantSeq FWD library preps.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3′ mRNA (FWD and REV)
    • Platform: Illumina

QuantSeq-Flex Second Strand Synthesis Module V2

With the QuantSeq-Flex Second Strand Synthesis Module V2 the random second strand synthesis primer can be substituted with a target-specific primer in the QuantSeq protocol. Please note that the second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need lower temperatures, please contact info@lexogen.com.

If dual-targeted RNA-Seq (custom primers in first and second strand synthesis) is required, please contact support@lexogen.com to request an optimized product.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq FWD
    • Platform: Illumina

Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina

Additional Modules for CORALL Kits:

PCR Add-on and Reamplification Kit V2 for Illumina (Cat. No. 208)

The PCR Add-on and Reamplification Kit V2 contains a PCR Mix, a thermostable polymerase, Illumina P5 and P7 primers without barcodes for 96 qPCR reactions. By adding SYBR Green I to the PCR reaction, a qPCR assay can be set up to determine the optimal number of cycles for the endpoint PCR of your QuantSeq V2, QuantSeq-Pool, or CORALL V2 cDNA. ATTENTION: use of this kit is not recommended for qPCR assays with previous versions of QuantSeq or CORALL.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too low library yield, while overcycling can lead to distortions in gene expression values.

In addition, the kit contains a reamplification primer mix (RE) that can be used instead of P5 and P7 primers to reamplify barcoded final libraries to increase the yield for sequencing or after use of enrichment panels. This kit is recommended for library reamplification for all Lexogen library prep kits.

  • Kit size: 96 qPCR rxns or 96 re-amplification rxns.
  • Compatibility information:
    ATTENTION: The qPCR assay is recommended for use with QuantSeq with UDI V2, QuantSeq-Pool, and CORALL RNA-Seq V2. Do not use this kit for qPCR assays with previous versions of QuantSeq or CORALL!
    Library reamplification is recommended for all Lexogen library prep kits.
  • Product
    • qPCR:
      • CORALL RNA-Seq V2 with UDI (Cat. No. 171 – 186),
      • QuantSeq V2 with UDI (Cat. No. 191 – 196),
      • QuantSeq-Pool (Cat. No. 139).
    • Library reamplification:
      • All CORALL library prep kits (all versions),
      • All QuantSeq library prep kits (all versions),
      • QuantSeq-Pool,
      • LUTHOR library prep, and
      • Small RNA-Seq Library prep.
    • Platform: Illumina

Download the User Guide from the Documents section.

Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina

Additional Modules for the Small RNA-Seq Kit:

Gel Extraction Module

The Gel Extraction Module enables the size selection of Small RNA-Seq libraries in a convenient spin column format. It prevents unwanted sequences (e.g., linker-linker artifacts and longer library fragments) dominating the NGS results and helps focus the sequencing depth on the desired microRNA fraction.

The module includes a reagent box consisting of Loading Dye, NGS MW Ladder, and NGS Control Ladder, and a purification box with all necessary buffers and purification columns for clean-up.

  • Kit size: 24 extractions
  • Compatibility information
    • Product: Small RNA-Seq Library Prep Kit

Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina

Additional Modules for the Teloprime Kit:

TeloPrime PCR Add-on Kit V2

The TeloPrime PCR Add-on Kit contains 16 reactions of the PCR Mix, the polymerase and the Forward and Reverse Primer separately. Based on this primers can be easily exchanged with gene-specific primers. The kit can furthermore be used to run multiple PCR reactions of your sample to generate sufficient template for any downstream application.

  • Kit size: 16 rxn
  • Compatibility information
    • Product: TeloPrime

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