Additional Modules for QuantSeq Kit:

PCRaddonkitillumina_550
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PCR Add-on Kit for Illumina

The PCR Add-on kit contains a PCR Mix including an Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode referred to as Barcode 00 for 96 PCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay to determine the optimal number of cycles for the endpoint PCR of your QuantSeq or CORALL cDNA libraries can be determined.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values.

The kit furthermore contains a reamplification primer that can be used to reamplify already barcoded libraries if they were undercycled to get enough material for sequencing.

NOTE: By 17.02.2017 the protocol was adjusted to be compatible with the QuantSeq upgrade, which includes the renaming of BC00 to P7 Primer 7000.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina

pdf PCR Add-on Kit for Illumina Instruction Manual – update 03.07.2019

UMIs_Module-550px
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UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1)

The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Cat. No. 081.96) contains the UMI Second Strand Synthesis Mix (USS). This mix simply replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq FWD Kit. The module allows unique tagging of individual transcripts with 6 nt long Unique Molecular Identifiers (UMI) located between the partial P5 adapter and the random priming sequence. Automated data analysis for QuantSeq FWD-UMI libraries is available on the Bluebee® Genomics Platform.

Use this module to identify PCR duplicates and eliminate amplification bias.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3‘ mRNA-Seq FWD
    • Platform: Illumina
Globin_Block_Module_500
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Globin Block Modules for QuantSeq

The Globin Block (RS-Globin Block) Modules for QuantSeq prevent the generation of library fragments from globin mRNAs, by blocking their extension during second strand synthesis. The module is compatible only with QuantSeq 3’ mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, and REV, Cat. No. 016), and is intended for the preparation of libraries from blood RNA samples.

Each module provides a modified RNA Removal Solution (RS-Globin Block) containing species-specific globin blocking oligos. The RS-Globin Block solution replaces the RNA Removal Solution (RS) from the standard QuantSeq 3’ mRNA-Seq Library Prep Kits. Separate modules are available for human (Homo sapiens) and pig (Sus scrofa).

The RS-Globin Block, Homo sapiens module (RS-GBHs, Cat. No. 070.96) is designed for human blood RNA and contains blocking oligos designed against the human alpha and beta hemoglobin chain mRNAs: HBA1, HBA2, and HBB.

The RS-Globin Block, Sus scrofa module (RS-GBSs, Cat. No. 071.96) is designed for pig blood RNA and contains blocking oligos designed against the pig alpha and beta hemoglobin chain mRNAs: HBA and HBB.

NOTE: The Globin Block Modules are also compatible with QuantSeq Kits released prior to the kit upgrade on 7th February, 2017. Please see the QuantSeq FAQs for details.

  • Kit Size: 96 rxn
  • Compatibility information:
    • Product: QuantSeq 3′ mRNA (FWD and REV)
    • Platform: Illumina

pdf Globin Block Modules Instruction Manual – release 19.12.2017

QSFlexfirststrand
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QuantSeq-Flex First Strand Synthesis Module

The QuantSeq-Flex First Strand Synthesis Module enables the exchange of the reverse transcription primer with a target specific primer in the QuantSeq protocol. It can furthermore be used to generate longer libraries with the standard QuantSeq 3’mRNA-Seq kit if needed.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq FWD
    • Platform: Illumina
QSFlexsecondstrand
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QuantSeq-Flex Second Strand Synthesis Module V2

With the QuantSeq-Flex Second Strand Synthesis Module V2 the random second strand synthesis primer can be substituted with a target-specific primer in the QuantSeq protocol. Please note that the second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need lower temperatures, please contact info@lexogen.com.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq FWD
    • Platform: Illumina
i56ntDualIndexing_550
Buy i5 6 nt Dual Indexing Kit (5001-5004)
Buy i5 6 nt Unique Dual Indexing Kit (5001-5096)

Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

The Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) contains perfectly balanced i5 indices. It is available in sets of 4 (5001-5004) and 96 (5001-5096) indices.

i5 (Index 2) indices can be introduced at the PCR step of CORALL (Cat. No. 095 and 096), SENSE mRNA-Seq V2 for Illumina (Cat. No. 001), SENSE Total RNA-Seq for Illumina (Cat. No. 009), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the i5 indices can be combined with any of the 96 i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 9,216 different barcoding options.

  • Kit size: 24 rxn or 96 rxn for each i5 index (5001-5004) and 1 rxn for each i5 index (5001-5096)
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina
  • Kit’s features
    • 96 i5 unique barcode combinations for fully-flexible dual indexing
    • 6 bp indices with perfect, uniform nucleotide and color balance
    • Hamming distance of 3 enables both error detection and error correction
    • Detect and quantify index hopping
    • Avoid cross-contamination and minimize index mis-assignment
    • Online index balance checker tool to assist with the choice of the optimal index combination for your experiment

pdf Lexogen i5 6 nt Dual Indexing Add-on Kits (5001-5096) Instruction Manual

BarcodeSenseQuantSeq_550
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Lexogen i7 6 nt Index Set (7001-7096)

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or CORALL kit for Illumina.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096).

pdf  Lexogen for Illumina Index Primer Overview

Purificationmodule
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Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / SENSE mRNA / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina / Ion Torrent
PCRaddonkitiontorrent
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PCR Add-on Kit for Ion Torrent

The PCR Add-on kit contains a PCR Mix including Ion Torrent specific primer and a thermostable polymerase. With the PCR Add-on kit more endpoint PCRs can be run for your sample to determine the exact number of cycles needed to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values. If your sample is undercycled the kit can furthermore be used to add more cycles and reamplify your library.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: QuantSeq 3’mRNA-Seq
    • Platform: Ion Torrent

Additional Modules for CORALL Kit:

PCRaddonkitillumina_550
Buy from our webstore

PCR Add-on Kit for Illumina

The PCR Add-on kit contains a PCR Mix including an Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode referred to as Barcode 00 for 96 PCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay to determine the optimal number of cycles for the endpoint PCR of your QuantSeq or CORALL cDNA libraries can be determined.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values.

The kit furthermore contains a reamplification primer that can be used to reamplify already barcoded libraries if they were undercycled to get enough material for sequencing.

NOTE: By 17.02.2017 the protocol was adjusted to be compatible with the QuantSeq upgrade, which includes the renaming of BC00 to P7 Primer 7000.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina

pdf PCR Add-on Kit for Illumina Instruction Manual – update 03.07.2019

i56ntDualIndexing_550
Buy i5 6 nt Dual Indexing Kit (5001-5004)
Buy i5 6 nt Unique Dual Indexing Kit (5001-5096)

Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

The Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) contains perfectly balanced i5 indices. It is available in sets of 4 (5001-5004) and 96 (5001-5096) indices.

i5 (Index 2) indices can be introduced at the PCR step of CORALL (Cat. No. 095 and 096), SENSE mRNA-Seq V2 for Illumina (Cat. No. 001), SENSE Total RNA-Seq for Illumina (Cat. No. 009), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the i5 indices can be combined with any of the 96 i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 9,216 different barcoding options.

  • Kit size: 24 rxn or 96 rxn for each i5 index (5001-5004) and 1 rxn for each i5 index (5001-5096)
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina
  • Kit’s features
    • 96 i5 unique barcode combinations for fully-flexible dual indexing
    • 6 bp indices with perfect, uniform nucleotide and color balance
    • Hamming distance of 3 enables both error detection and error correction
    • Detect and quantify index hopping
    • Avoid cross-contamination and minimize index mis-assignment
    • Online index balance checker tool to assist with the choice of the optimal index combination for your experiment

pdf Lexogen i5 6 nt Dual Indexing Add-on Kits (5001-5096) Instruction Manual

BarcodeSenseQuantSeq_550
Buy from our webstore

Lexogen i7 6 nt Index Set (7001-7096)

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or CORALL kit for Illumina.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096).

pdf  Lexogen for Illumina Index Primer Overview

Purificationmodule
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Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / SENSE mRNA / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina / Ion Torrent

Additional Modules for SENSE Kit:

PCRaddonkitillumina_550
Buy from our webstore

PCR Add-on Kit for Illumina

The PCR Add-on kit contains a PCR Mix including an Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode referred to as Barcode 00 for 96 PCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay to determine the optimal number of cycles for the endpoint PCR of your QuantSeq or CORALL cDNA libraries can be determined.

The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values.

The kit furthermore contains a reamplification primer that can be used to reamplify already barcoded libraries if they were undercycled to get enough material for sequencing.

NOTE: By 17.02.2017 the protocol was adjusted to be compatible with the QuantSeq upgrade, which includes the renaming of BC00 to P7 Primer 7000.

  • Kit size: 96 rxn
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina

pdf PCR Add-on Kit for Illumina Instruction Manual – update 03.07.2019

i56ntDualIndexing_550
Buy i5 6 nt Dual Indexing Kit (5001-5004)
Buy i5 6 nt Unique Dual Indexing Kit (5001-5096)

Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

The Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) contains perfectly balanced i5 indices. It is available in sets of 4 (5001-5004) and 96 (5001-5096) indices.

i5 (Index 2) indices can be introduced at the PCR step of CORALL (Cat. No. 095 and 096), SENSE mRNA-Seq V2 for Illumina (Cat. No. 001), SENSE Total RNA-Seq for Illumina (Cat. No. 009), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the i5 indices can be combined with any of the 96 i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 9,216 different barcoding options.

  • Kit size: 24 rxn or 96 rxn for each i5 index (5001-5004) and 1 rxn for each i5 index (5001-5096)
  • Compatibility information
    • Product: CORALL, SENSE mRNA-Seq V2 and QuantSeq
    • Platform: Illumina
  • Kit’s features
    • 96 i5 unique barcode combinations for fully-flexible dual indexing
    • 6 bp indices with perfect, uniform nucleotide and color balance
    • Hamming distance of 3 enables both error detection and error correction
    • Detect and quantify index hopping
    • Avoid cross-contamination and minimize index mis-assignment
    • Online index balance checker tool to assist with the choice of the optimal index combination for your experiment

pdf Lexogen i5 6 nt Dual Indexing Add-on Kits (5001-5096) Instruction Manual

BarcodeSenseQuantSeq_550
Buy from our webstore

Lexogen i7 6 nt Index Set (7001-7096)

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or CORALL kit for Illumina.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096).

pdf  Lexogen for Illumina Index Primer Overview

sense barcode kit
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SENSE mRNA-Seq Barcode Kits for Ion Torrent

96 external barcodes for the SOLiD and 24 in-line barcodes for the Ion Torrent system are available for the SENSE mRNA-Seq library prep kit. Each Barcode Set contains 12 different barcodes with 8 or 24 rxn.

  • Kit size: 8 or 24 rxn
  • Compatibility information
    • Product: SENSE mRNA-Seq
    • Platform: Ion Torrent
Purificationmodule
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Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / SENSE mRNA / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina / Ion Torrent
Automationmodulesense
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Automation Module for SENSE mRNA-Seq V2

The Automation Module for SENSE mRNA-Seq is part of the autoSENSE package. Together with the SENSE mRNA-Seq V2 kit and the autoSENSE software it provides a fully automated walk-away mRNA-Seq library preparation protocol and the possibility to select for larger library sizes.

  • Kit size: 96 preps
  • Compatibility information
    • Product: SENSE mRNA-Seq V2
    • Platform: Illumina

Additional Modules for the Small RNA-Seq Kit:

GelExtractionModule_500
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Gel Extraction Module

The Gel Extraction Module enables the size selection of Small RNA-Seq libraries in a convenient spin column format. It prevents unwanted sequences (e.g., linker-linker artifacts and longer library fragments) dominating the NGS results and helps focus the sequencing depth on the desired microRNA fraction.

The module includes a reagent box consisting of Loading Dye, NGS MW Ladder, and NGS Control Ladder, and a purification box with all necessary buffers and purification columns for clean-up.

  • Kit size: 24 extractions
  • Compatibility information
    • Product: Small RNA-Seq Library Prep Kit
Purificationmodule
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Purification Module with Magnetic Beads

The Purification Module with Magnetic Beads contains all the necessary reagents to carry out additional purifications e.g., after PCR or to concentrate the libraries if needed.

  • Kit size: 200 rxn

  • Compatibility information
    • Product: CORALL / SENSE mRNA / QuantSeq all products / TeloPrime / Small RNA-Seq Kit
    • Platform: Illumina / Ion Torrent

Additional Modules for TeloPrime Kit:

pcraddonkitteloprime
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TeloPrime PCR Add-on Kit V2

The TeloPrime PCR Add-on Kit contains 16 reactions of the PCR Mix, the polymerase and the Forward and Reverse Primer separately. Based on this primers can be easily exchanged with gene-specific primers. The kit can furthermore be used to run multiple PCR reactions of your sample to generate sufficient template for any downstream application.

  • Kit size: 16 rxn
  • Compatibility information
    • Product: TeloPrime