The Gel Extraction Module enables the size selection of Small RNA-Seq libraries in a convenient spin column format. It prevents unwanted sequences (e.g., linker-linker artifacts and longer library fragments) dominating the NGS results and helps focus the sequencing depth on the desired microRNA fraction.
The module includes a reagent box consisting of Loading Dye, NGS MW Ladder, and NGS Control Ladder, and a purification box with all necessary buffers and purification columns for clean-up.
- 24 extractions
- Product: any small RNA-Seq library prep kit, e.g., Lexogen’s Small RNA-Seq Library Prep Kit for Illumina (Cat. No. 052)
- Gels: 6 % non-denaturing polyacrylamide gels recommended
- Reagent Box
- Buffers, Gel Filtration, and Purification Columns