Support Tools Overview
The Index Balance Checker tool will help you select the optimal index combination for multiplexed sequencing. Specifically, the applet evaluates the color balance of your chosen Lexogen i7 Indices (Lexogen i7 6 nt Index Set) for all CORALL, QuantSeq, and SENSE kits and can be used on any number of samples for any Illumina machine (HiSeq, NextSeq, MiSeq, MiniSeq).
Galaxy SIRV suite is a set of software tools accompanying the Spike-in RNA Variant Control Mixes (SIRVs) by Lexogen. It allows you to design and evaluate your SIRV experiment, and to compare it to other similar experiments. The SIRV Suite streamlines and unifies the data evaluation process.
After sequencing, demultiplexing can be carried out by the standard Illumina pipeline. All Lexogen i7 and i5 index sequences are available for download.
In addition to the standard error-correction included in the Illumina pipeline, Lexogen’s Index Error Correction Tool (available free of charge) can be used in order to rescue even more reads.