Support Tools Overview
RiboCop is offered as a stand-alone kit with the option to choose from three optimized Probe Mixes for depletion of rRNA from mixed bacterial samples (META, Cat. No. 125) or from Gram negative or Gram positive bacteria (G- or G+, Cat. No. 126 and 127, respectively) grown in monoculture.
If you are uncertain which kit to choose of RiboCop for Bacteria, then the RiboCop for Bacteria Kit Selection Tool can help to find the best-suited kit for your species of interest.
Libraries should ideally be pooled in an equimolar ratio for multiplexed sequencing. It is important to ensure accurate quantification of the individual libraries prior to pooling, as well as for the library pool (lane mix). To quantify your libraries measure the concentration using either qPCR or fluorescence-based assays and determine the average library size, using microcapillary electrophoresis analysis (e.g., Bioanalyzer or Fragment Analyzer). The Library Quantification File will calculate the molarities of each library from the concentration measurement and average size. Further it will provide the volumes of each library to be used for preparation of an equimolar lane mix.
The Index Balance Checker tool will help you select the optimal index combination for multiplexed sequencing. Specifically, the applet evaluates the color balance of your chosen Lexogen i7 Indices (Lexogen i7 6 nt Index Set) for all CORALL, QuantSeq, and SENSE kits and can be used on any number of samples for any Illumina machine (HiSeq, NextSeq, MiSeq, MiniSeq).
Includes videos with tips for Lexogen Magnetic Bead Purification and tips for QuantSeq First Strand Synthesis.
Easily determine the volumes you need to mix when pooling samples together in the QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina kit (Cat. No. 139), thanks to our user-friendly calculator.
This tool also provides you with recommended input range (which depends on your pooling strategy) as well as a calculation for >56 samples (pool splitting).