Support Tools Overview
RiboCop is offered as a stand-alone kit with the option to choose from three optimized Probe Mixes for depletion of rRNA from mixed bacterial samples (META, Cat. No. 125) or from Gram negative or Gram positive bacteria (G- or G+, Cat. No. 126 and 127, respectively) grown in monoculture.
If you are uncertain which kit to choose of RiboCop for Bacteria, then the RiboCop for Bacteria Kit Selection Tool can help to find the best-suited kit for your species of interest.
The Index Balance Checker tool will help you select the optimal index combination for multiplexed sequencing. Specifically, the applet evaluates the color balance of your chosen Lexogen i7 Indices (Lexogen i7 6 nt Index Set) for all CORALL, QuantSeq, and SENSE kits and can be used on any number of samples for any Illumina machine (HiSeq, NextSeq, MiSeq, MiniSeq).
After sequencing, demultiplexing can be carried out by the standard Illumina pipeline. All Lexogen i7 and i5 index sequences are available for download.
In addition to the standard error-correction included in the Illumina pipeline, Lexogen’s Index Error Correction Tool (available free of charge) can be used in order to rescue even more reads.
Includes videos with tips for Lexogen Magnetic Bead Purification and tips for QuantSeq First Strand Synthesis.