Description
SPLIT Rapid Viral RNA/DNA Extraction Kit
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This product can be used for SARS-CoV-2 research. |
The SPLIT Rapid Viral RNA/DNA Extraction Kit enables the isolation of high-quality RNA & DNA from liquid samples using a fast, streamlined protocol. For the extraction of highly pure, gDNA-free RNA preparations also from difficult samples and for demanding applications please visit the SPLIT RNA Extraction Kit.
Fast, Simplified Protocol
The protocol enables the isolation of viral RNA in only 15 minutes, and 24 samples can be completed in 25 minutes. Since the workflow is based on sample lysis and column purification, only a standard centrifuge is required. No phenol extraction is needed, reducing the exposure of the user to chemical hazards and avoiding the production of toxic waste.
High-Quality Viral RNA and DNA
The kit yields high-quality RNA suitable for reverse transcription, RT-qPCR, and targeted RNA-Seq. RNases are immediately activated when the sample is resuspended in the Isolation Buffer. For the analysis of viral RNA, the co-isolated genomic DNA does not have to be removed, and the DNA itself can be analyzed in other applications.
Validated for SARS-CoV-2 RNA Extraction
The kit has been validated externally for extraction of SARS-CoV-2 RNA with buccal swab samples in virus transport medium (VTM). At Lexogen, we have confirmed that spike-in viral RNA can be extracted from media such as HBSS and PBS. The kit is intended for use in research and development. Validation is required for integration into a specific diagnostic workflow.
Kit Size and High-Throughput Option
The kit is available in a 400-reaction version and in bulk format. Further, the protocol is transferable to magnetic beads for plate-based, automatable high-throughput RNA extraction. For further details and support please contact support@lexogen.com.
Workflow
The SPLIT Rapid Viral RNA/DNA Extraction Kit contains reagents for the isolation of total RNA from 400 liquid samples. A bulk reagent option is also available.
FAQ
Frequently Asked Questions
Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact support@lexogen.com.
The kit is designed for fluid samples and has been validated with buccal swab samples in virus transport medium.
The extraction takes only 15 minutes, and 24 samples can be finished in about 25 minutes.
Only a standard (non-refrigerated) centrifuge is need (in addition to standard laboratory equipment such as pipettes etc). The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.
Only ethanol and isopropanol have to be supplied by the user. The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.
The recovered RNA is suitable for targeted RNA-Seq; for more demanding applications such as whole transcriptome analysis (total RNA-Seq) we recommend using the SPLIT RNA Extraction Kit.
No, the genomic DNA is present in the final eluate but does not affect analysis of viral RNA in targeted qRT-PCR and targeted RNA-Seq applications.
Downloads
SPLIT Rapid Viral RNA/DNA Extraction Kit
User Guide – release 30.06.2020
Send us your publication & get the RNA T-shirt!
Product Flyer – release 30.06.2020