SPLIT Rapid Viral RNA/DNA Extraction Kit
- Isolation of high-quality RNA from fluid samples
- Rapid 15-minute protocol validated for viral applications
- Inhibitor-free RNA ready-to-use in RT-qPCR and targeted RNA-Seq assays
Description
SPLIT Rapid Viral RNA/DNA Extraction Kit
The SPLIT Rapid Viral RNA/DNA Extraction Kit enables the isolation of high-quality RNA & DNA from liquid samples using a fast, streamlined protocol. For the extraction of highly pure RNA for demanding applications, please visit the SPLIT RNA Extraction Kit.
Fast, Simplified Protocol
The protocol enables the isolation of viral RNA in only 15 minutes, and 24 samples can be completed in 25 minutes. Since the workflow is based on sample lysis and column purification, only a standard centrifuge is required. No phenol extraction is needed, reducing the exposure of the user to chemical hazards and avoiding the production of toxic waste.
High-Quality Viral RNA and DNA
The kit yields high-quality RNA suitable for reverse transcription, RT-qPCR, and targeted RNA-Seq. RNases are immediately deactivated when the sample is resuspended in the Isolation Buffer. For the analysis of viral RNA, the co-isolated genomic DNA does not have to be removed, and the DNA itself can be analyzed in other applications.Validated for SARS-CoV-2 RNA Extraction
The kit has been validated externally for extraction of SARS-CoV-2 RNA with buccal swab samples in virus transport medium (VTM). At Lexogen, we have confirmed that spike-in viral RNA can be extracted from media such as HBSS and PBS. The kit is intended for use in research and development. Validation is required for integration into a specific diagnostic workflow.Kit Size and High-Throughput Option
The kit is available in a 400-reaction version and in bulk format. Further, the protocol is transferable to magnetic beads for plate-based, automatable high-throughput RNA extraction. For further details and support please contact support@lexogen.com.
Workflow
The SPLIT Rapid Viral RNA/DNA Extraction Kit contains reagents for the isolation of total RNA from 400 liquid samples. A bulk reagent option is also available.
30 min
The sample is lysed in a highly chaotropic
isolation buffer which facilitates complete
solubilization and guarantees RNase inhibition.
FAQ
Frequently Asked Questions
Access our frequently asked question (FAQ) resources via the buttons below.
Please also check our General Guidelines and FAQ resources!
How do you like the new online FAQ resource? Please share your feedback with us!
Downloads
Safety Data Sheet
If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.
Purchase Information Available on Request
Please contact us for more information and purchase inquiries at sales@lexogen.com.