Description

SPLIT Rapid Viral RNA/DNA Extraction Kit

This product can be used for SARS-CoV-2 research.

The SPLIT Rapid Viral RNA/DNA Extraction Kit enables the isolation of high-quality RNA & DNA from liquid samples using a fast, streamlined protocol. For the extraction of highly pure, gDNA-free RNA preparations also from difficult samples and for demanding applications please visit the SPLIT RNA Extraction Kit.

Fast, Simplified Protocol

The protocol enables the isolation of viral RNA in only 15 minutes, and 24 samples can be completed in 25 minutes. Since the workflow is based on sample lysis and column purification, only a standard centrifuge is required. No phenol extraction is needed, reducing the exposure of the user to chemical hazards and avoiding the production of toxic waste.

High-Quality Viral RNA and DNA

The kit yields high-quality RNA suitable for reverse transcription, RT-qPCR, and targeted RNA-Seq. RNases are immediately activated when the sample is resuspended in the Isolation Buffer. For the analysis of viral RNA, the co-isolated genomic DNA does not have to be removed, and the DNA itself can be analyzed in other applications.

Validated for SARS-CoV-2 RNA Extraction

The kit has been validated externally for extraction of SARS-CoV-2 RNA with buccal swab samples in virus transport medium (VTM). At Lexogen, we have confirmed that spike-in viral RNA can be extracted from media such as HBSS and PBS. The kit is intended for use in research and development. Validation is required for integration into a specific diagnostic workflow.

Kit Size and High-Throughput Option

The kit is available in a 400-reaction version and in bulk format. Further, the protocol is transferable to magnetic beads for plate-based, automatable high-throughput RNA extraction. For further details and support please contact support@lexogen.com.

Workflow

The SPLIT Rapid Viral RNA/DNA Extraction Kit contains reagents for the isolation of total RNA from 400 liquid samples. A bulk reagent option is also available.

Column-Based Purification

Step 1:

The sample is lysed in a highly chaotropic isolation buffer which
facilitates complete solubilization and guarantees RNase inhibition.

Column-Based Purification

Step 2:

Isopropanol is added to the sample before loading onto
a silica micro-spin column.

Column-Based Purification

Step 3:

RNA and DNA bind specifically to the column,
while most of the other components are in the flow-through.

Column-Based Purification

Step 4:

Contaminants such as PCR inhibitors, divalent cations,
and proteins are removed in three wash steps.

Column-Based Purification

Step 5:

10-50 µl Elution Buffer is added to the column to elute pure viral RNA.

Extraction of RISC-associated Small RNAs

Step 6:

The RNA extraction is finished, and the RNA is ready for downstream
applications such as qRT-PCR and targeted RNA-Seq.

FAQ

Frequently Asked Questions

Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact support@lexogen.com.

1. For which sample type is this kit suitable?

The kit is designed for fluid samples and has been validated with buccal swab samples in virus transport medium.

2. How long does the protocol take?

The extraction takes only 15 minutes, and 24 samples can be finished in about 25 minutes.

3. Which equipment is needed for the protocol?

Only a standard (non-refrigerated) centrifuge is need (in addition to standard laboratory equipment such as pipettes etc). The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.

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