Description

SPLIT Rapid Viral RNA/DNA Extraction Kit

The SPLIT Rapid Viral RNA/DNA Extraction Kit enables the isolation of high-quality RNA & DNA from liquid samples using a fast, streamlined protocol. For the extraction of highly pure, gDNA-free RNA preparations also from difficult samples and for demanding applications please visit the SPLIT RNA Extraction Kit.

Fast, Simplified Protocol

The protocol enables the isolation of viral RNA in only 15 minutes, and 24 samples can be completed in 25 minutes. Since the workflow is based on sample lysis and column purification, only a standard centrifuge is required. No phenol extraction is needed, reducing the exposure of the user to chemical hazards and avoiding the production of toxic waste.

High-Quality Viral RNA and DNA

The kit yields high-quality RNA suitable for reverse transcription, RT-qPCR, and targeted RNA-Seq. RNases are immediately activated when the sample is resuspended in the Isolation Buffer. For the analysis of viral RNA, the co-isolated genomic DNA does not have to be removed, and the DNA itself can be analyzed in other applications.

Validated for SARS-CoV-2 RNA Extraction

The kit has been validated externally for extraction of SARS-CoV-2 RNA with buccal swab samples in virus transport medium (VTM). At Lexogen, we have confirmed that spike-in viral RNA can be extracted from media such as HBSS and PBS. The kit is intended for use in research and development. Validation is required for integration into a specific diagnostic workflow.

Kit Size and High-Throughput Option

The kit is available in a 400-reaction version and in bulk format. Further, the protocol is transferable to magnetic beads for plate-based, automatable high-throughput RNA extraction. For further details and support please contact support@lexogen.com.

Workflow

The SPLIT Rapid Viral RNA/DNA Extraction Kit contains reagents for the isolation of total RNA from 400 liquid samples. A bulk reagent option is also available.

Column-Based Purification
Step 1:
The sample is lysed in a highly chaotropic isolation buffer which
facilitates complete solubilization and guarantees RNase inhibition.
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Column-Based Purification
Step 2:
Isopropanol is added to the sample before loading onto
a silica micro-spin column.
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Column-Based Purification
Step 3:
RNA and DNA bind specifically to the column,
while most of the other components are in the flow-through.
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Column-Based Purification
Step 4:
Contaminants such as PCR inhibitors, divalent cations,
and proteins are removed in three wash steps.
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Column-Based Purification
Step 5:
10-50 µl Elution Buffer is added to the column to elute pure viral RNA.
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Extraction of RISC-associated Small RNAs
Step 6:
The RNA extraction is finished, and the RNA is ready for downstream
applications such as qRT-PCR and targeted RNA-Seq.
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FAQ

Frequently Asked Questions

Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact support@lexogen.com.

The kit is designed for fluid samples and has been validated with buccal swab samples in virus transport medium.

The extraction takes only 15 minutes, and 24 samples can be finished in about 25 minutes.

Only a standard (non-refrigerated) centrifuge is need (in addition to standard laboratory equipment such as pipettes etc). The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.

Only ethanol and isopropanol have to be supplied by the user. The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.

The recovered RNA is suitable for targeted RNA-Seq; for more demanding applications such as whole transcriptome analysis (total RNA-Seq) we recommend using the SPLIT RNA Extraction Kit.

The Kit provides reagents to isolate RNA from 200 µl liquid swab sample. Upscaling will decrease the number of reactions that can be processed with the provided material.

No, the genomic DNA is present in the final eluate but does not affect analysis of viral RNA in targeted qRT-PCR and targeted RNA-Seq applications.

Downloads

SPLIT Rapid Viral RNA/DNA Extraction Kit

pdf  User Guide – release 30.06.2020
pdf  Product Flyer – release 30.06.2020

Material Safety Datasheets

MSDS Information can be found in the Documents page.

Purchase Information Available on Request

Please contact us for more information and purchase inquiries at sales@lexogen.com.