SPLIT Rapid Viral RNA/DNA Extraction Kit
The SPLIT Rapid Viral RNA/DNA Extraction Kit enables the isolation of high-quality RNA & DNA from liquid samples using a fast, streamlined protocol. For the extraction of highly pure, gDNA-free RNA preparations also from difficult samples and for demanding applications please visit the SPLIT RNA Extraction Kit.
The SPLIT Rapid Viral RNA/DNA Extraction Kit contains reagents for the isolation of total RNA from 400 liquid samples. A bulk reagent option is also available.
facilitates complete solubilization and guarantees RNase inhibition.
a silica micro-spin column.
while most of the other components are in the flow-through.
and proteins are removed in three wash steps.
applications such as qRT-PCR and targeted RNA-Seq.
Frequently Asked Questions
Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact firstname.lastname@example.org.
The kit is designed for fluid samples and has been validated with buccal swab samples in virus transport medium.
The extraction takes only 15 minutes, and 24 samples can be finished in about 25 minutes.
Only a standard (non-refrigerated) centrifuge is need (in addition to standard laboratory equipment such as pipettes etc). The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.
Only ethanol and isopropanol have to be supplied by the user. The full list of user-supplied consumables and equipment is listed in the User Guide for SPLIT Rapid Viral RNA/DNA Extraction Kit on page 7.
The recovered RNA is suitable for targeted RNA-Seq; for more demanding applications such as whole transcriptome analysis (total RNA-Seq) we recommend using the SPLIT RNA Extraction Kit.
No, the genomic DNA is present in the final eluate but does not affect analysis of viral RNA in targeted qRT-PCR and targeted RNA-Seq applications.