Demultiplexing and Error Correction Tool – iDemux
iDemux is an all-in-one command line tool available for download on github and pypi. iDemux allows users to perform:
- i1 demultiplexing of QuantSeq-Pool libraries
- i5 and i7 demultiplexing of QuantSeq, QuantSeq-Pool, CORALL (Total and mRNA-Seq), and LUTHOR libraries
- Error correction of above mentioned demultiplexed reads when using Lexogen’s 12 nt Unique Dual Indexing (UDI) System.
After sequencing, demultiplexing can be carried out by the standard Illumina pipeline. All Lexogen i7 and i5 index sequences are available for download here.
In addition to the standard error-correction included in the Illumina pipeline, iDemux can also be used to rescue even more reads when utilizing Lexogen’s 12 nt UDI System.
Please contact firstname.lastname@example.org for further information or if you have questions pertaining to your data analysis workflow.