Broad Species and Sample Compatibility
RiboCop rRNA Depletion Kits are available for Human/Mouse/Rat (Cat. No. 144), Human/Mouse/Rat plus Globin (Cat. No. 145), and for Bacteria (Cat. No. 125 – 127). For RiboCop HMR compatibility with other species, please consult the FAQs, or contact firstname.lastname@example.org. For RiboCop for Bacteria, a dedicated Selection Tool is available.
Efficient Elimination of rRNA
RiboCop allows efficient removal of rRNAs. RiboCop for Human/Mouse/Rat (HMR, Cat. No. 144) removes cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse, and rat samples. RiboCop for Human/Mouse/Rat plus Globin (HMR+Globin, Cat. No. 145) depletes both rRNA and globin mRNA from blood samples. RiboCop for Bacteria (META, G-, G+, Cat. No. 125 – 127) removes 23S, 16S, and 5S rRNA from Gram positive and Gram negative bacterial species as well as from mixed bacterial samples. Sequencing depth is thus preserved for relevant RNA types.
The RiboCop protocol takes 1.5 hours to complete (30 minutes hands-on time). No enzymatic reactions or mechanical shearing steps are involved, leaving full-length transcripts intact for downstream processing.
Flexible Input Requirements
RiboCop is suitable for rRNA depletion of 1 – 1,000 ng total RNA input amounts and is compatible with intact or degraded RNA (including FFPE) samples. Specific input recommendations apply for certain sample types and are described in the respective User Guides.
Magnetic beads are used for depletion and purification steps rendering the protocol completely automation-friendly.
Various Downstream Applications
RiboCop rRNA Depletion Kits are offered as stand-alone kits in different sizes for use with any downstream application. For convenience, RiboCop for Human/Mouse/Rat Kits (HMR and HMR+Globin) are also available in combination with the CORALL Total RNA-Seq Library Prep Kit (Cat. No. 146 and 147).