RiboCop rRNA Depletion KitLexogen
RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat)
After extraction total RNA comprises large amounts of ribosomal RNA (rRNA) which frequently is of little interest. Lexogen’s RiboCop rRNA Depletion Kit enables removal of rRNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.
Efficient Elimination of rRNA
RiboCop allows efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Thus, sequencing depth is preserved for relevant RNA species.
The RiboCop protocol takes 2 hours to complete. No enzymatic reactions or mechanical shearing steps are involved, leaving full-length transcripts intact for downstream processing.
Flexible Input Requirements
RiboCop is suitable for rRNA depletion of 1 – 1000 ng total RNA input amounts from intact or degraded RNA (including FFPE) samples.
Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.
Various Downstream Applications
Samples are treated using a set of affinity probes for specific depletion of rRNA sequences. RiboCop probes efficiently remove rRNA and therefore afford a comprehensive view of transcriptome composition.
Denaturation of total RNA
Affinity probes and total RNA are mixed and denatured.
Hybridization of affinity probes to rRNA
Denatured total RNA and probes are hybridized.
Removal of bound rRNA molecules
Depletion beads are conditioned and used to remove probes
along with hybridized ribosomal RNA from solution.
Purification of depleted RNA
The depleted RNA is purified for downstream processing.
Frequently Asked Questions
Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact email@example.com.
RiboCop contains probes for the rRNA depletion of human, mouse and rat RNA samples and was also successfully tested on hamster, mini pig, and zebrafish samples.
Depending on input sample, standard recovery rate yield varies from 1 – 3%.
Depending on input sample, depletion down to 1 – 2% is standard.
80% EtOH is required. All other reagents are provided in the kit.
RiboCop is suitable for input amounts from 1 ng – 1 µg of total RNA input.
If you don´t have a thermomixer available you can just run it on a thermocycler without agitation.
In RiboCop V1.2 a new Hybridization Solution (HS) is introduced that enables a significant increase of the sample input (up to 26 µl of total RNA). The rpm-values and reaction volumes got furthermore adjusted to make the kit compatible with high throughput applications.
User Guide – update 01.08.2016 (Release of RiboCop rRNA Depletion Kit V1.2)
Material Safety Datasheets
MSDS information for RiboCop rRNA Depletion Kit V1.2
RiboCop Free Trial Kit Form
If you would like to receive a RiboCop Free Trial Kit, please fill in the form.
If you have any questions, please do not hesitate to contact us at firstname.lastname@example.org or +43 (0) 1 345 1212 – 41.