RiboCop for Human/Mouse/Rat V2
Total RNA from mammalian species is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for ~80 – 90 % of all RNA. Lexogen’s RiboCop rRNA Depletion Kits for Human/Mouse/Rat remove undesired cytoplasmic (28S, 18S, 5.8S, and 45S rRNA) and mitochondrial rRNA (mt16S, mt12S, and 5S) from intact as well as degraded material, including formalin-fixed, paraffin-embedded (FFPE) samples.
RiboCop for Human/Mouse/Rat (HMR) V2 is offered as a stand-alone (Cat. No. 144) or as a bundled version with the CORALL Total RNA-Seq Library Prep Kit (Cat. No. 146). RiboCop Kits are compatible with all random primed total RNA library prep kits.
Robust Performance Over a Wide Range of Input Amounts
Ribosomal RNA was depleted from Universal Human Reference RNA (UHRR) using RiboCop for Human/Mouse/Rat (HMR) V2 over a wide range of input amounts (1 ng to 1 µg). RiboCop HMR V2 efficiently reduces rRNA reads to below 1% (Fig. 1) while maintaining unbiased transcript abundance (Fig. 2).
Figure 2 | RiboCop maintains unbiased expression profiles while efficiently removing undesired ribosomal RNA from UHRR. Correlation of transcript abundance in untreated samples vs. samples depleted with RiboCop for Human/Mouse/Rat (HMR) V2 for 1 ng – 1 µg UHRR. Libraries were prepared and sequenced as described in Figure 1. Reads were mapped against the GRCh38.95 reference genome using STAR aligner and counted with FeatureCounts (including multi mappers).