TeloPrime Full-Length cDNA Amplification Kit V2
- Full-length cDNA generation with high yield
- Exceptional 5‘ cap specificity
- Ideal for long-read NGS library generation
(e.g. PacBio™ and Oxford Nanopore™) - 1 ng – 2 μg total RNA input
- Flexible protocol enables use of custom primers
for reverse transcription
Description
TeloPrime Full-Length cDNA Amplification Kit V2
This product can be used for SARS-CoV-2 research.
The TeloPrime Full-Length cDNA Amplification Kit V2 is an all-in-one protocol for generating full-length cDNA from total RNA.
Full-Length cDNA Synthesis
Based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, it is highly selective for full-length RNA molecules that are both capped and polyadenylated.
For Multiple Downstream Applications
The full-length cDNA products can be used for various downstream applications such as NGS, RACE, cloning, microarray probes, and normalization. It enables the detection and correct quantification of splice variants and their true transcription start- and end-sites, in both short and long mRNA molecules. For further full-length or gene-specific PCRs, Lexogen offers a TeloPrime PCR Add-on Kit V2 (Cat.No.018.16) containing 16 rxn. This kit contains the PCR Forward and Reverse Primer separately, hence they can be alternatively substituted with the gene-specific primer of interest.
Full-Length cDNA Synthesis
Based on the CDLL technology the ligation of the 5’ linker to the 3’ end of the cDNA takes place in a highly cap-dependent manner, providing an exceptional cap-specificity and eliminating cDNA products from degraded mRNA.
In an mRNA-Seq experiment, mRNAs were tagged at their 5’ end using TeloPrime, Template-Switch (TS) or Oligo Capping (OC). Transcript start sites (TSS) were mapped to the mouse genome. The accumulated TSS read coverage is plotted versus the normalized annotated transcript length to show relative TSS mapping for the top 500 expressed genes.
Workflow
The TeloPrime Full-Length cDNA Amplification Kit V2 is a protocol for generating full-length cDNA from total RNA. It is based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, and is highly selective for full-length RNA molecules that are both capped and polyadenylated.
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