Poly(A) RNA Selection Kit
The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.
Highly Specific for Poly(A) RNA
Figure 1. Bioanalyzer traces of the poly(A) RNA enrichment using 5 µg of Universal Human Reference RNA (UHRR) (A) input with the Poly(A) RNA Selection Kit. Recovery of poly(A) selected UHRR (B) is about 2 % of the initial input amount.
are aliquoted and washed.
are hybridized to oligodT beads.
and tRNA,will not be captured by the oligodT beads
and will be washed away.
by heating in water.
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In the traces below you’ll see that the Bioanalyzer software classifies two peaks within the trace of a poly(A) selected sample as 18S and 28S rRNA (Figure A). However, the overlay with diluted total RNA clearly demonstrates that those peaks are not rRNA peaks (Figure B). Poly(A) selection was successful and no rRNA was left. This was also confirmed by NGS sequencing.
Figure A. Bioanalyzer trace after the Poly(A) RNA Selection using 5 µg of Universal Human Reference RNA (UHRR). The Bioanalyzer software wrongly classifies two peaks within the trace of the poly(A) selected sample as 18S and 28S rRNA.
Figure B. Overlay of Bioanalyzer traces before and after the Poly(A) RNA Selection. The overlay with diluted total RNA clearly demonstrates that those peaks identified as rRNA (in A) are not rRNA peaks.
Red trace: diluted total Universal Human Reference RNA (UHRR), intact (RIN 8.5). Blue trace: poly(A) RNA selected RNA.
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