Nurture Growth, Harvest Discoveries
Unveil the Complete Plant
Transcriptome with RiboCop
RiboCop for Plants
Plant total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for up to ~97 % of all transcripts. Lexogen’s RiboCop rRNA Depletion Kits for Plants remove undesired cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and plastid (4.5S, 5S, 16S and 23S) rRNA from intact as well as degraded input RNA. Ribo-depletion affords an unbiased view of the transcriptome, including non-coding and non-polyadenylated RNA species.
RiboCop for Plants is ideally suited for Next Generation Sequencing (NGS) approaches in combination with CORALL RNA-Seq V2 Library Preparation Kits. RiboCop Kits are also compatible with other random primed total RNA library prep kits.
Performance
Robust Performance Over a Wide Range of Input Amounts
Ribosomal RNA was depleted from Arabidopsis thaliana total RNA using RiboCop for Plants over a wide range of input amounts (1 ng to 1 µg). RiboCop for Plants efficiently reduces rRNA reads for all input amounts (Fig. 1).
Figure 1 | RiboCop rRNA Depletion for Plants efficiently removes rRNA across a wide range of input amounts. NGS libraries were prepared using Lexogen’s CORALL RNA-Seq V2 Library Prep Kit. Successful depletion was monitored by NGS sequencing (NextSeq2000, 1×90 bp) and subsequent analysis of remaining rRNA reads from 10 ng untreated (Total RNA) and depleted A. thaliana RNA (1 ng, 100 ng, and 1 µg). The percentage of reads mapping to rRNA is plotted in blue.
Increased Gene Detection upon rRNA Depletion
Discover the complete plant transcriptome and sequence what matters to your experiment. RiboCop for Plants allows an unobstructed view of the transcriptome and increases gene detection at 3 M reads / sample by more than 45 % through efficient rRNA removal (Fig. 2).
Figure 2 | Increased gene detection upon rRNA depletion. A. thaliana RNA samples were depleted with RiboCop for Plants using either 1 µg or 1 ng input RNA. Libraries were prepared using Lexogen’s CORALL RNA-Seq V2 Library Prep Kit and sequenced on Illumina NextSeq2000 (1×90 bp). Reads were mapped against the A. thaliana reference genome using STAR aligner and counted with FeatureCounts.
RiboCop for Plants Performance across Species
RiboCop enriches RNAs of interest across a range of different plant species (Fig. 3) and is suitable for depletion of various crops including corn (Zea mays), soybean (Glycine max), and others.
Figure 3 | RiboCop rRNA Depletion for Plants efficiently removes rRNA from A.thaliana, Z. mays, and G. max. NGS libraries were prepared using Lexogen’s CORALL RNA-Seq V2 Library Prep Kit. Successful depletion was monitored by NGS sequencing (NextSeq2000, 1×90 bp) and subsequent analysis of remaining rRNA reads from 5 ng untreated (Total RNA). 50 ng RNA from A. thaliana, Z. mays, and G. max were used for rRNA depletion prior to library generation. Reads were mapped to the respective reference genomes and the percentage of reads mapping to rRNA is plotted in blue.
Workflow
Samples are treated using a set of affinity probes for specific depletion of rRNA sequences. RiboCop probes efficiently remove rRNA and therefore afford a comprehensive view of transcriptome composition.
FAQ
Frequently Asked Questions
Access our frequently asked question (FAQ) resources via the buttons below.
Please also check our General Guidelines and FAQ resources!
How do you like the new online FAQ resource? Please share your feedback with us!
Downloads
Safety Data Sheet
If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.
First time user of RiboCop?
First Time User? We’re excited to offer you an exclusive introductory offer.
Buy from our Webstore
Need a web quote?
You can generate a web quote by Register or Login to your account. In the account settings please fill in your billing and shipping address. Add products to your cart, view cart and click the “Generate Quote” button. A quote in PDF format will be generated and ready to download. You can use this PDF document to place an order by sending it directly to sales@lexogen.com.
Web quoting is not available for countries served by our distributors. Please contact your local distributor for a quote.
