The PCR Add-on and Reamplification Kit V2 contains a PCR Mix, a thermostable polymerase, Illumina P5 and P7 primers without barcodes for 96 qPCR reactions. By adding SYBR Green I to the PCR reaction a qPCR assay can be set up to determine the optimal number of cycles for the endpoint PCR of your QuantSeq V2, QuantSeq-Pool, or CORALL V2 cDNA. ATTENTION, use of this kit is not recommended for qPCR assays with previous versions of QuantSeq or CORALL.
The qPCR assay is recommended to determine the exact number of cycles for the endpoint PCR in order to prevent any under- or overcycling of your library. Undercycling may result in too low library yield, while overcycling can lead to distortions in gene expression values.
In addition, the kit contains a reamplification primer mix (RE) that can be used instead of P5 and P7 primers to reamplify barcoded final libraries to increase the yield for sequencing or after use of enrichment panels. This kit is recommended for library reamplification of all Lexogen library prep kits.
This kit is not compatible for use with the following products:
This kit is not recommended for qPCR assays with older versions of QuantSeq 3’ mRNA-Seq and CORALL RNA-Seq.