Description
Not sure which CORALL RNA-Seq product is right for you?
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CORALL RNA-Seq V2 is Lexogen’s new stranded RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99 %). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites. All CORALL RNA-Seq V2 Kits and bundles are delivered with Lexogen UDI 12 nt Unique Dual Indexing Sets for Unique Dual Indexing.
Features and Application:
CORALL is suitable for all whole transcriptome RNA-seq applications, including:
- Gene expression profiling
- Isoform discovery and quantification
- Alternative splicing studies
- Transcript (re)annotation
- SLAMseq metabolic RNA sequencing
System Compatibility:
- HiSeq 2000/2500/3000/4000
- NextSeq 500/550/2000
- MiSeq
- iSeq
- NovaSeq 6000
Kit Contents:
- CORALL Library Generation Module
- Library Amplification Module
- Purification Module with Magnetic Beads
- Lexogen UDI 12 nt Unique Dual Indexing Set (indices UDI12A_0001-0024, UDI12A_0001-0096, UDI12A_0097-0192, UDI12A_0193-0288, UDI12A_0283-0384, UDI12A_0001-0384, or UDI12B_0001-0024, UDI12B_0001-0096, for Cat. No. 171.24, 171.96, 172.96, 173.96, 174.96, 176.384, or 175.24 and 175.96, respectively.)
CORALL mRNA-Seq bundles additionally contain a Poly(A) RNA Selection Kit.
CORALL Total RNA-Seq bundles with RiboCop additionally contain a RiboCop rRNA Depletion Kit either for Human/Mouse/Rat or for Human/Mouse/Rat Plus Globin.
Recommended! PCR Add-on and Reamplification Kit V2 (96 rxns)
The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.
