QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina

//QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina

QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina

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NOTE: The QuantSeq-Pool kit includes 96 Sample-Barcodes, which are sufficient for multiplexing of up to 96 samples. If multiplexing of more than 96 samples is required, additional i5 and/or i7 indices are required. We recommend using Lexogen’s UDI 12 nt Unique Dual Indexing Set B1 (UDI12B_0001-0096).

SKU: 139.96 Category:

Description

QuantSeq offers an all-in-one library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
QuantSeq-Pool is a streamlined solution for gene expression profiling for large screening projects using early pooling and batch processing and is easily scalable from a few to 36,864 samples.

Features and Application:

  • Gene expression studies
  • From as low as 10 ng of total RNA

Kit Contents:

  • i1 12 nt RT-Set for QuantSeq-Pool (96-well plate, dried-in)
  • QuantSeq Library Generation Module
  • QuantSeq-Pool Library Amplification Module
  • Purification Module

System Compatibility:

  • NovaSeq 6000
  • HiSeq 2000/2500/3000/4000
  • NextSeq 500/550 (only FWD)
  • MiSeq
  • MiniSeq
  • Genome Analyzer

Optional i5/i7 Unique Dual Indexing (96 UDIs)

The Lexogen UDI 12 nt Unique Dual Indexing Sets contain 96 pre-mixed UDIs, which are fully compatible with QuantSeq-Pool libraries. One UDI primer pair is sufficient for i5/i7 barcoding of up to 96 sample-barcoded QuantSeq-Pool libraries. Using the full set of 96 UDI primer pairs up to 9,216 QuantSeq-Pool libraries can be multiplexed on one sequencing lane.

Product Items

Catalog Nr. Product Name
139.96 QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina, 96 preps
105.96 Lexogen UDI 12 nt Set B1 (UDI12B_0001-0096), 1 rxn/UDI

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