The Globin Block (RS-Globin Block) Modules for QuantSeq prevent the generation of library fragments from globin mRNAs, by blocking their extension during second strand synthesis. The module is compatible only with QuantSeq 3’ mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, and REV, Cat. No. 016), and is intended for the preparation of libraries from blood RNA samples.
Each module provides a modified RNA Removal Solution (RS-Globin Block) containing species-specific globin blocking oligos. The RS-Globin Block solution replaces the RNA Removal Solution (RS) from the standard QuantSeq 3’ mRNA-Seq Library Prep Kits. Separate modules are available for human (Homo sapiens) and pig (Sus scrofa).
The RS-Globin Block, Homo sapiens module (RS-GBHs, Cat. No. 070.96) is designed for human blood RNA and contains blocking oligos designed against the human alpha and beta hemoglobin chain mRNAs: HBA1, HBA2, and HBB.
The RS-Globin Block, Sus scrofa module (RS-GBSs, Cat. No. 071.96) is designed for pig blood RNA and contains blocking oligos designed against the pig alpha and beta hemoglobin chain mRNAs: HBA and HBB.
NOTE: The Globin Block Modules are also compatible with QuantSeq Kits released prior to the kit upgrade on 7th February, 2017. Please see the QuantSeq FAQs for details.
Kit Size: 96 rxn
- Product: QuantSeq 3′ mRNA (FWD and REV)
- Platform: Illumina