SIRVs
The most comprehensive
RNA spike-in controls

A publicly-available command line tool, SIRVsuite, can be used to QC an RNA-Seq workflow using Lexogen’s SIRVs. The SIRVsuite features 3 main modules:

1. Coverage Module – compares observed spike-in gene coverage to the expected coverage, which is estimated from transcripts
2. ERCC Correlation Module – validates expected vs measured ratios for single-transcript spike-ins in the whole concentration range
3. SIRV Concentration Module – detects anomalies of SIRV transcript concentration among samples or the overall spiked-in transcript distribution

To get started with SIRVsuite, please visit Lexogen’s GitHub page: https://github.com/Lexogen-Tools/SIRVsuite.