A publicly-available command line tool, SIRVsuite, can be used to QC an RNA-Seq workflow using Lexogen’s SIRVs. The SIRVsuite features 3 main modules:
- Coverage Module – compares observed spike-in gene coverage to the expected coverage, which is estimated from transcripts
- ERCC Correlation Module – validates expected vs measured ratios for single-transcript spike-ins in the whole concentration range
- SIRV Concentration Module – detects anomalies of SIRV transcript concentration among samples or the overall spiked-in transcript distribution
To get started with SIRVsuite, please visit Lexogen’s GitHub page: https://github.com/Lexogen-Tools/SIRVsuite.