Workflow

Samples are treated using a set of affinity probes for specific depletion of rRNA sequences. RiboCop probes efficiently remove rRNA and therefore afford a comprehensive view of transcriptome composition.

Denaturation of total RNA
Step 1:
Affinity probes and total RNA are mixed and denatured.
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Hybridization of affinity probes to rRNA
Step 2:
Denatured total RNA and probes are hybridized.
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Removal of bound rRNA molecules
Step 3:
Depletion beads are conditioned and used
to remove probes along with hybridized
ribosomal RNA from solution.
Timer_RiboCop_V1.3
Purification of depleted RNA
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Step 4:
The depleted RNA is purified for downstream
processing.
Timer_RiboCop_V1.3