Workflow
Samples are treated using a set of affinity probes for specific depletion of rRNA sequences. RiboCop probes efficiently remove rRNA and therefore afford a comprehensive view of transcriptome composition.
Denaturation of total RNA
Step 1:
Affinity probes and total RNA are mixed and denatured.
Hybridization of affinity probes to rRNA
Step 2:
Denatured total RNA and probes are hybridized.
Removal of bound rRNA molecules
Step 3:
Depletion beads are conditioned and used
to remove probes along with hybridized
ribosomal RNA from solution.
to remove probes along with hybridized
ribosomal RNA from solution.
Purification of depleted RNA
Step 4:
The depleted RNA is purified for downstream
processing.
processing.