Ribosomal RNA Depletion
(noun) /rɑ́jbəwsə́wməl RNA dɪplɪ́jʃən/
Total RNA is comprised of large amounts of ribosomal RNA (rRNA) which can make up between ~80 – 98 % of all RNA molecules in a sample. Ribosomal RNA depletion removes these undesired transcripts to access transcripts of interest. Ultimately, rRNA depletion enables detection of all types of non-ribosomal RNAs, including coding mRNAs and non-coding transcripts such as snoRNAs, snRNAs, and lncRNAs. Thereby, ribosomal depletion affords an undisturbed view of an organism’s complete transcriptome.
RiboCop rRNA Depletion Kits
Total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA). RiboCop enables very efficient removal of rRNA. Besides ribosomal RNA, RiboCop can also deplete globin mRNA, which makes up 30 – 80 % of all mRNA in mammalian blood. Thus, RiboCop focuses sequencing reads on RNA of interest while maintaining unbiased expression profiles.
New! RiboCop rRNA Depletion for Yeast affords an unbiased view of the yeast transcriptome and allows analysis of non-coding and non-polyadenylated RNAs.
RiboCop Kits are available for:
- Yeast,
- Human/Mouse/Rat rRNA depletion,
- Combined Human/Mouse/Rat rRNA and globin mRNA depletion, and for
- Gram negative, Gram positive or mixed bacterial samples.
Benefits
Sequence what matters most! RiboCop rRNA Depletion is a well-established, enzyme-free, and automation-friendly rRNA depletion kit for various species, including human, mouse, rat and bacterial samples. RiboCop offers exceptional performance on low input samples, down to 1 ng starting amount and is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples.
Preserve your RNA!
Ideal for challenging samples
Maximize unique sequencing reads
Avoid depletion bias
Unlock low input samples
Process Blood Samples with Ease
Applications for RiboCop rRNA Depletion
- Expression analysis, incl. non-coding transcripts
- Analysis of long non-coding RNA, snRNAs, snoRNAs, and circRNAs
- Isoform analysis, incl. non-polyadenylated RNAs
- FFPE and Biobank transcriptomics
- Biomarker analysis, e.g., circRNAs in blood
- Transcript annotation, incl. de novo assembly of non-coding transcripts
- Ribo-depletion prior to long-read sequencing
- Bacterial transcriptome research
- Metatranscriptomics
- Host-pathogen studies
- Yeast transcriptomics