Description
QuantSeq FFPE is based on the trusted QuantSeq 3’ mRNA-Seq technology and is optimized for and validated on FFPE samples. QuantSeq FFPE represents the most cost-efficient solution for gene expression profiling of FFPE samples and is, with its 3’ mRNA-Seq approach, the only kit of its kind available on the market. With built-in Unique Molecular Identifiers (UMIs) and an optimized protocol for FFPE and degraded samples, QuantSeq FFPE shows enhanced performance even with low input amounts (less than 10 ng). QuantSeq FFPE enables streamlined generation of Illumina-compatible libraries in less than 4.5 hours and delivers robust and reliable gene expression profiling results from polyadenylated transcripts. All QuantSeq FFPE 3’ mRNA-Seq Kits are delivered with Lexogen UDI 12 nt Unique Dual Indexing Sets for Unique Dual Indexing.
Features and Application:
- Cost-efficient gene expression profiling
- FFPE and degraded RNA (low RIN, including low DV200)
- Low inputs (down to 10 ng)
- Built-in Unique Molecular Identifiers (UMIs) for best practise
System Compatibility:
- iSeq 100
- MiniSeq
- NextSeq 500 – 2000
- HiSeq 2000, 2500, 3000, 4000
- NovaSeq 6000 (v1.0 and 1.5 reagent kits)
- NovaSeq X
- Element Biosciences AVITI*
- Singular Genomics G4*
*contact support@lexogen.com for details about sequencing platform compatibility.
Kit Contents:
QuantSeq FFPE with UDI kits are provided with four essential components:
- QuantSeq FFPE Library Generation Module
- Library Amplification Module
- Purification Module with Magnetic Beads
- Lexogen UDI 12 nt Unique Dual Indexing Set packed in single reaction plates for 24, 96, or 384 reactions (Indices UDI12A_0001-0024, UDI12A_0001-0096, UDI12A_0001-0384, or UDI12B_0001-0024, UDI12B_0001-0096)
Recommended! PCR Add-on and Reamplification Kit V2 (96 rxns)
The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.
