Description
CORALL FFPE Whole Transcriptome RNA-Seq is Lexogen’s new stranded RNA library prep kit with excellent whole transcriptome coverage specifically validated on FFPE samples. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99 %). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies which are ideal for processing FFPE samples and already degraded RNA. All CORALL FFPE Whole Transcriptome RNA-Seq Kits and bundles are delivered with Lexogen UDI 12 nt Unique Dual Indexing Sets for Unique Dual Indexing.
Features and Application:
CORALL FFPE is suitable for all whole transcriptome RNA-seq applications, including:
- Transcript expression profiling of FFPE samples
- Isoform discovery and quantification
- Alternative splicing, fusion, and variant analysis
System Compatibility:
- HiSeq 2000/2500/3000/4000
- NextSeq 500/550/2000
- MiSeq
- iSeq
- NovaSeq 6000 / X
- Element Biosciences AVITI*
- Singular Genomics G4*
*contact support@lexogen.com for details about sequencing platform compatibility.
Kit Content:
- CORALL FFPE Library Generation Module
- Library Amplification Module
- Purification Module with Magnetic Beads
- Lexogen UDI 12 nt Unique Dual Indexing Set (indices UDI12A_0001-0024, UDI12A_0001-0096, UDI12A_0001-0384, or UDI12B_0001-0024, UDI12B_0001-0096)
CORALL FFPE Whole Transcriptome RNA-Seq bundles with RiboCop additionally contain a RiboCop rRNA Depletion Kit for Human/Mouse/Rat.
