Description
The Lexogen UDI 12 nt Unique Dual Indexing Add-on Kits contain:
- 96 or 384 pre-mixed UDIs, packaged in single reactions
- PCR buffer and enzyme
For use of the 12 nt UDIs with Lexogen kits, please check out the available bundle options. Two 96-well plate format index sets are available: Sets A1 – A4 for Workflow A and Set B1 for Workflow B (see Table below).
| Illumina Instruments | Workflow | UDI Set |
|---|---|---|
| NovaSeq™ 6000 (v1.0 reagent kits) HiSeq® 3000/4000 (SR) HiSeq® 2000/2500 MiSeq® | A | A1, A2, A3, A4 |
| NovaSeq™ 6000 (v1.5 reagent kits) NextSeq™ 500/550 HiSeq® 3000/4000 (PE) MiniSeq™ 6000 iSeq™ 100 | B | B1* |
* Sets A1 to A4 can be used for workflow B, but sequences are reversed (to be specified during data analysis).
With the UDIs Add-on kits UDIs are introduced at the PCR step of following library preparation kits: QuantSeq 3’ mRNA-Seq FWD and REV (Cat. No. 015 and 016), CORALL Total RNA-Seq (Cat. No. 095), and others. For further questions, please contact support@lexogen.com.
The UDI 12 nt Unique Dual Indexing Sets are already included in the QuantSeq-FWD V1 (Cat. No. 113 – 115 and 129 – 131), QuantSeq FWD V2 (Cat. No. 191-196), CORALL V1 (Cat. No. 117 – 119 and 132 – 134), and CORALL V2 (Cat. No. 171 – 176) with UDI kits.
- Kit features:
- Up to 384 Unique Dual Indices (UDIs) for high-level multiplexing
- 12 nt long indices with read-out options of 12, 10, and 8 nt
- Optimized sets for different workflows on Illumina machines
- Maximal inter-index distance for each sample size enables efficient error correction
- Demultiplexing and Error Correction Tool – iDemux
- Maximizing sequencing data output
- Kit size: 96 or 384 rxns of each UDI (1 rxn/UDI)
- Compatibility with other library preparation kits:
- Any RNA and DNA sequencing library prep methods utilizing TruSeq™ – compatible stubby adapters (where partial Illumina adapters are introduced during the workflow and completed with the index information during the library amplification step).
Please reach out to us for more information: support@lexogen.com
- Any RNA and DNA sequencing library prep methods utilizing TruSeq™ – compatible stubby adapters (where partial Illumina adapters are introduced during the workflow and completed with the index information during the library amplification step).
