Description
LUTHOR High-Definition Single-Cell 3’ mRNA-Seq kits are provided with all necessary reagents to perform the full library preparation protocol:
- Cell lysis (optional)
- THOR reaction
- In vitro transcription (IVT)
- Reverse transcription
- Endpoint PCR
- Purification reactions, with magnetic beads
Cat. No. 221 additionally provides the UDI Set B1, designed to perform error correction and maximize the reads recovered from your sequencing run.
LUTHOR HD kits are also compatible with Lexogen’s UDI Sets A1 to A4, available as separate products, with the following catalog numbers:
- 101.96: Lexogen UDI 12 nt Set A1 (UDI12A_0001-0096), 1 rxn/UDI
- 102.96: Lexogen UDI 12 nt Set A2 (UDI12A_0097-0192), 1 rxn/UDI
- 103.96: Lexogen UDI 12 nt Set A3 (UDI12A_0193-0288), 1 rxn/UDI
- 104.96: Lexogen UDI 12 nt Set A4 (UDI12A_0289-0384), 1 rxn/UDI
- 156.384: Lexogen UDI 12 nt Sets A1-A4 (UDI12A_0001-0384), 1 rxn/UDI
Features and Application:
- Gene expression studies
- Single isolated cells, ultra-low RNA inputs (down to 10 pg)
- LIVE-Seq or similar technologies with “less-than-a-cell” inputs (less than 10 pg)
System Compatibility:
Illumina platforms
- iSeq 100
- MiniSeq
- NextSeq 500 – 2000
- HiSeq 2000, 2500, 3000, 4000
- NovaSeq 6000 (v1.0 and 1.5 reagent kits)
- NovaSeq X
Element Biosciences platforms
- Aviti
We recommend the PCR Add-on and Reamplification Kit V2 , 96 rxns (Cat. No. 208)
The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.
