Description
QuantSeq with UDI V2 kits are provided with four essential components:
- a library generation module (same as the original QuantSeq FWD 015)
- a library amplification module
- a library purification module (magnetic beads)
- a plate containing 24 or 96 Unique Dual Indices (UDI), packaged in single reactions
- for the 384-reaction kit: four plates containing 384 different UDI (sets A1 to A4)
UDI Set B1 is designed for Illumina chemistries of iSeq 100, MiniSeq, NextSeq 500 – 2000, HiSeq 3000 and 4000, as well as NovaSeq 6000 (v1.5 reagent kits).
Sets A1 to A4 can also be used for the above instruments, but sequences are reversed (this needs to be specified during data analysis).
Features and Application:
- Gene expression studies
- Degraded RNA (low RIN, high DV200) such as FFPE
- Low inputs (down to 1ng)
System Compatibility:
- iSeq 100
- MiniSeq
- NextSeq 500 – 2000
- HiSeq 2000, 2500, 3000, 4000
- NovaSeq 6000 (v1.0 and 1.5 reagent kits)
Recommended! PCR Add-on and Reamplification Kit V2 (96 rxns)
The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.