Cell-to-cell variability is a fundamental feature among multi-cellular organisms, which contain highly diverse cell types with unique transcriptome profiles. Sequencing of individual cells allows assessing heterogeneity in gene expression even between cells of similar type or origin. The requirement for thousands to millions of cells to compensate for inefficient library conversion limits the usability of these methods for cells that are notoriously hard to isolate in large quantities, and for studying perturbations on a cell-to-cell level.
In this webinar you will learn about the novel LUTHOR 3’ mRNA-Seq methodology providing the first high resolution single cell protocol that allows in-depth analysis of individual cells without restricting the analysis to the highest abundant transcripts. LUTHOR combines a revolutionary RNA amplification technology with highly efficient 3’ library preparation for RNA-Seq from single cells with unprecedented sensitivity. Lexogen’s proprietary THOR (T7 High-resolution Original RNA) Amplification technology generates RNA copies directly from the endogenous mRNA pool without requiring a double-stranded template cDNA as intermediate.
The combination of the innovative RNA Amplification and the highly efficient library generation technologies offers unprecedented sensitivity for ultra-low input and single cells, with up to ~15,000 genes detected from one single cell at 1 M reads (CPM >1).