Lexogen now offers CORALL mRNA-Seq bundles including poly(A) selection providing a convenient complete workflow solution for mRNA-Seq library preparation.
CORALL mRNA-Seq features an easy all-in-one workflow from total RNA to ready-to-sequence libraries and takes less than 6 hours to complete. The kit is applicable for a wide range of total RNA input (from 1 ng to 1 µg) and comes with Unique Molecular Identifiers (UMIs) and Unique Dual Indexing (UDIs).
UMIs are introduced during CORALL library generation and enable the removal of PCR duplicates for precise quantification.
CORALL mRNA-Seq Kits are available with up to 384 pre-mixed UDIs for Illumina’s Forward Strand (A) and Reverse Complement (B) workflows (Set A1-A4: Cat. No. 158-161, 163, or Set B1: Cat. No. 162). Lexogen’s new 12 nt UDIs feature superior error correction for maximal sequencing data output especially at high-level multiplexing, for example, on NovaSeq® instruments.
Reads derived from CORALL libraries are highly strand-specific and correspond directly to the mRNA sequence. Furthermore, Read 1 contains the UMI information making it directly accessible in cost-efficient single-read sequencing mode. User-friendly data analysis pipelines are available on various platforms for CORALL users to explore their data – even without proficiency in bioinformatics.
The complete CORALL mRNA-Seq workflow is automation compatible and can hence be easily implemented in projects where high throughput sample processing is required.
CORALL mRNA-Seq as well as CORALL Total RNA-Seq (in combination with ribosomal RNA depletion) offer excellent gene detection and transcript coverage even for low RNA input amounts (between 1-10 ng) which are in line or outperform other leading protocols on the market.