Lexogen releases a new PCR Add-on and Reamplification Kit V2 that combines reagents for qPCR and library reamplification in one convenient kit. Ensure high-quality results for your RNA-Seq workflow and rescue your precious, but low yield libraries.
The qPCR assay is the gold standard for optimizing NGS library amplification and ensuring the highest data quality for RNA-Seq experiments. This inexpensive assay ensures efficiency and consistency of the individual processing steps before moving to sequencing – the most cost-intensive part of the RNA-Seq workflow.
Using the qPCR assay to optimize NGS library amplifications ensures that:
The new PCR Add-on and Reamplification Kit V2 increases qPCR robustness and performance also with difficult library types, e.g., libraries derived from 3’ mRNA-Seq workflows which include homopolymer stretches notoriously difficult to amplify.
In addition, the new kit now contains reagents for library reamplification that can be used to rescue low yield libraries and salvage them for sequencing. Apart from low yield libraries, the kit is also ideally suited for library amplification following post-library prep enrichment steps with RNA- or DNA-Seq panels for Illumina (Fig. 1).
Figure 1 | PCR Add-on and Reamplification Kit V2 for Illumina is ideal for amplification following panel-based enrichment of NGS libraries.
The PCR Add-on and Reamplification Kit V2 was extensively validated for use together with Lexogen’s QuantSeq and CORALL RNA-Seq library prep technologies and is a perfect addition to complete your RNA-Seq workflow. Get yours today!
Learn more about qPCR and PCR cycle number determination and library preparation quality control from Lexicon – our free resources selection.
