SENSE Total RNA-Seq Library Prep Kit
SENSE Total RNA-Seq is a strand-specific library prep kit for accurate gene expression profiling, whole transcriptome sequencing, discovery, and quantification of antisense transcripts and overlapping genes.
The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts by omitting reverse transcription artifacts, and therefore avoids spurious second strand cDNA synthesis, which may result in the detection of false antisense transcription. Thereby an exceptional (>99.9 %) strand-specificity and reduced experimental noise is reached, enabling the detection and quantification of antisense transcripts with high confidence.
With the up to 96 i7 Index Primer included in the kit libraries can be easily multiplexed. Four additional i5 Index Primer available in the i5 Dual Indexing Add-on Kit (Cat. No. 047) allow for an even higher degree of unique indexing and multiplexing of up to 384 samples.
Streamlined Ribosomal RNA Depletion
For efficient utilization of RNA-Seq reads highly abundant ribosomal RNA should be eliminated prior library preparation. SENSE Total RNA-Seq Kit is available in combination with RiboCop rRNA Depletion Kit. This is a complete solution for RNA-Seq library preparation, starting with total RNA and finishing with a ready-to-sequence library.
Flexible Input Requirements
Libraries for Different Sequencing Read Length
The SENSE Total RNA-Seq kit is based on Lexogen’s proprietary strand-displacement stop/ligation technology and needs poly(A) selected or rRNA depleted RNA input.
heterodimer mix to the poly(A) selected or rRNA depleted RNA.
to the next hybridized heterodimer, where the newly synthesized cDNA insert
is ligated to the stopper.
starter/stopper binding sites.
converted to double-stranded DNA. The double-stranded library is purified
using magnetic beads to adjust the library length and get rid of second
strand synthesis reaction components.
sequences required for cluster generation and to produce sufficient
material for quality control and sequencing. i7 and i5 indices can be
added during this step in order to be able to uniquely multiplex your
samples for the sequencing run.
can interfere with quantification.
For viewing the whole workflow on page please click here
Frequently Asked Questions
Please find a list of the most frequently asked questions below. If you cannot find the answer to your question here or want to know more about our products, please contact firstname.lastname@example.org.
SENSE Total RNA-Seq Library Prep Kit for Illumina
User Guide for intact RNA or partially degraded RNA – update 17.02.2017 (Introduction of new i7 Index Plate; referral to i5 Dual Indexing Add-on Kit; qPCR endpoint determination using only 1.7 µl template and set to 50 % FU)
User Guide for heavily degraded or FFPE samples – update 17.02.2017 (Introduction of new i7 Index Plate; referral to i5 Dual Indexing Add-on Kit; qPCR endpoint determination using only 1.7 µl template and set to 50 % FU)
PCR Add-on Kit for Illumina Instruction Manual – update 17.02.2017 (Renaming of BC00 to i7 Primer 7000; qPCR endpoint determination using only 1.7 µl template and set to 50 % FU)
i5 Dual Indexing Add-on Kit for QuantSeq/SENSE Instruction Manual – update 17.02.2017 (Inclusion of QuantSeq-Flex; Renaming of i7 indices in i7 Index Plate)
SENSE Total RNA-Seq for Illumina Index Primer Overview (i7 Index Plate) – for kits bought after 17.02.2017
Barcode Plate Overview – for kits bought before 17.02.2017
Material Safety Datasheets
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