The new QuantSeq with UDI V2 kits are proposed as a series of 3’ mRNA-Seq library preparation kits including Lexogen’s patented 12 nucleotide-long Unique Dual Indices (UDI), in five different sets (A1, A2, A3, A4 and B1). These sets are identical to the ones of the original version 1. With the constant increase in sequencing throughput capacities, UDI have now become an essential component of most RNA-Seq libraries, providing the ability to pool and subsequently identify each library during the analysis step. At Lexogen, we made sure to bring scientists the utmost design quality with our ultrapure, single-reaction packaged, optimally designed UDI sets.
For more information about each set, please consult our dedicated FAQ webpage.
If you already have a library preparation kit without UDI (QuantSeq Flex, Reverse, Forward or Pool) our UDI Add-on V2 modules are also available for purchase separately. They are delivered together with an improved PCR system, which can be seamlessly adapted to previous QuantSeq kits. Our modules are also compatible with library preparation kits from other vendors. For more information, please reach out to our specialists at email@example.com.
QuantSeq with UDI V2 kits can of course still be used with the existing modules:
- PCR Add-on Kit for Illumina 020.96 – to determine the number of amplification cycles, by qPCR
- Reamplification Add-on Kit 080.96 – to reamplify undercycled libraries (on-demand kit)
- QuantSeq UMI module 081.96 – to add UMI to your libraries
- Blocking modules to prevent amplification of over-represented RNA sequences:
Human globin: 070.96
Pig globin: 071.96
Mouse BC1: 167.96