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CORALL Total RNA-Seq Library Prep Kit

  • Complete coverage of transcripts from start to end
  • Ready-to-sequence libraries within 4.5 hours
  • Unique Molecular Identifiers (UMIs) seamlessly included
  • Excellent protocol-inherent strandedness (>99 %)
  • 1 ng to 1 µg of total RNA input
  • Easy all-in-one protocol
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RiboCop rRNA Depletion Kit V1.3
(Human/Mouse/Rat)

  • Efficient elimination of cytoplasmic and mitochondrial rRNA
  • Simple workflow – no enzymatic reactions
  • Output ready for NGS library prep
  • Down to 1 ng of total RNA input
  • Faster protocol: rRNA depleted in only 1.5 hours
    (30 minutes hands-on time) new
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SPLIT RNA Extraction Kit for Blood NEW

  • Now also available for RNA extraction from blood
  • Efficient depletion of globin mRNA
  • High-quality RNA for demanding downstream applications
  • Total RNA or small and large RNA fractions
  • No DNase treatment – no RNA degradation
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TeloPrime Full-Length cDNA Amplification Kit V2

  • Full-length cDNA generation with high yield
  • Exceptional 5‘ cap specificity
  • Ideal for long-read NGS library generation
    (e.g. PacBio™ and Oxford Nanopore™)
  • 1 ng – 2 μg total RNA input
  • Flexible protocol enables use of custom primers
    for reverse transcription
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SLAMseq Metabolic RNA Labeling Kit for RNA-Seq

  • Analyze transcriptome-wide kinetics of RNA synthesis and turnover
  • Measure nascent RNA expression and transcript stability
  • Enhance the temporal resolution of differential expression
  • Identify primary and secondary transcriptional targets
  • No pull-down or biochemical isolation required
  • Use in combination with QuantSeq 3’ mRNA-Seq for cost-effective,
    high-throughput metabolic sequencing
  • SLAMdunk - automated and user-friendly SLAMseq-QuantSeq data analysis
    pipeline on the Bluebee ® genomics analysis platform
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Lexogen Services

  • QuantSeq 3’ mRNA-Seq library preparation
  • Cost-efficient, multiplexed sequencing
  • Data analysis and reporting
  • Fully integrated package, economical, and convenient
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HOME2019-10-21T14:33:00+01:00

Latest News

Welcome

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Lexogen is a biotech company focusing on RNA and complete transcriptome studies using next generation sequencing technologies.

We have been at the forefront of RNA research since 2007, and our technologies and products are being used by thousands of scientists all over the world. “Lexo” in Greek means “word”, and Lexogen stands for the word of genes, which is the transcriptome. We strive to be the pioneers in this fascinating field of RNA science, and together with the fastest-growing technology of the past years, the next generation sequencing (NGS), we are sure that we can make a big contribution to the world.

Our Products

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CORALL Total RNA-Seq Library Prep Kit

CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99%). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites.

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QuantSeq Expression Profiling Library Prep Kits

QuantSeq kits enable cost-efficient sequencing by counting. These kits are an exceptional alternative to standard RNA-Seq and microarrays. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple and accurate. Up to 384 samples can be multiplexed in one lane, saving your sequencing space. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.

QuantSeq 3′ mRNA-Seq Library Prep Kit FWD is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail. The kit is available for Illumina and Ion Torrent platforms.

QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.

QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.

New! Globin Block Modules for QuantSeq are now available for human (Homo sapiens, Cat. No. 070.96) and pig (Sus scrofa, Cat. No. 071.96), enabling fully-integrated globin depletion for blood RNA-Seq library preparation, using the QuantSeq 3’ mRNA-Seq Library Prep protocol. The RS-Globin Block solution simply replaces the standard RNA Removal Solution, no additional steps are added to the protocol.

RiboCop rRNA Depletion Kit V1.3 (Human/Mouse/Rat)

RiboCop rRNA Depletion Kit V1.3 (Human/Mouse/Rat) enables removal of ribosomal RNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.

New! Faster protocol: rRNA depleted in only 1.5 hours (30 minutes hands-on time).

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SLAMseq Metabolic RNA Labeling Kit for RNA-Seq

SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics.

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Small RNA-Seq Library Prep Kit

Small RNA-Seq Library Prep Kit provides a protocol for generation of small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.

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SIRVs (Spike-in RNA Variant Control Mixes)

The SIRVs are available as sets of transcripts designed to validate the performance of RNA sequencing workflows and to control individual samples passing through RNA-Seq experiments. They are available in 3 sets.

SIRV-Set 1 (Iso Mix E0, E1, E2) is made for a detailed validation of isoform-specific RNA-seq pipelines.

SIRV-Set 2 (Iso Mix E0) enables the straightforward assessment and control of isoform detection and quantification in each sample.

SIRV-Set 3 (Iso Mix E0 / ERCC) that combines isoforms and non-isoform ERCC transcripts provides controls for examining isoform resolution and dynamic range resolution.

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SENSE mRNA-Seq Library Prep Kits

SENSETM mRNA-Seq Library Prep Kits generate ready-to-sequence libraries with exceptional strand-specificity from low amounts of input RNA within a few hours. SENSE mRNA-Seq Library Prep Kits are available for Illumina and Ion Torrent sequencing platforms. An automated SENSE mRNA-Seq Library Prep can be performed on the PerkinElmer Sciclone/Zephyr NGS workstations.

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TeloPrime Full-Length cDNA Amplification Kit V2

TeloPrimeTM Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.

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SPLIT RNA Extraction Kit

SPLITTM RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into a large and a small RNA fraction, facilitating the analysis of e.g. mRNA and miRNA from the same sample. Thus the protocol is ideal for seamlessly preparing libraries for NGS of total RNA or its large and small fractions.

NEW! SPLIT RNA Extraction Kit for Blood for efficient depletion of globin mRNA during the extraction.

What our clients say

“We used the new CORALL kit for performing transcriptome-analysis of CRISPR-modified cells in order to understand the consequences of deregulated epigenetic modifiers. In our hands the kit performance was highly satisfying in terms of data-quality and reproducibility across biological replicates. It furthermore convinced us with the ease of use, clarity of instructions, details in the manual and handling of reagents.“
Dr. Max Koeppel,
Head of the Functional Tumor Genomics Group at Leibniz-Institute DSMZ, Germany
“We have successfully used the Lexogen QuantSeq FWD kit for the preparation of RNAseq libraries over the last year. The kit provides us with a highly cost effective and reliable, fast solution to generate RNAseq libraries that we subsequently use to assess gene expression levels and most importantly to rapidly compile and assess alternative cleavage and polyadenylation profiles. QuantSeq is a cornerstone for our RNAseq based research, in particular as multiplexing allows us to combine it with cost effective sequencing on the Ion Torrent platform.”
André Furger,
Associate Professor, Chromosomal and RNA Biology, Department of Biochemistry, University of Oxford, UK
“With Lexogen Poly(A) Selection kit I was able to isolate reasonable amount of polyadenylated RNA with no trace of rRNA contamination. I see great advantages of this kit in clear-cut protocol and quite low starting concentration of total RNA that you can easily scale-up based on chosen downstream application. The other thing that should be appreciated is Lexogen customer service that provides you with very high level of support.”
Květoslava Brožinová,
Research Technician, Research Group: ERA Chair – RNA and Immunity, CEITEC, Brno, Czech Republic