Unleash the Yeast!
Lexogen is a biotech company focusing on RNA and complete transcriptome studies using next generation sequencing technologies.
We have been at the forefront of RNA research since 2007, and our technologies and products are being used by thousands of scientists all over the world. “Lexo” in Greek means “word”, and Lexogen stands for the word of genes, which is the transcriptome. We strive to be the pioneers in this fascinating field of RNA science, and together with the fastest-growing technology of the past years, the next generation sequencing (NGS), we are sure that we can make a big contribution to the world.
QuantSeq kits enable cost-efficient sequencing by counting. These kits are an exceptional alternative to standard RNA-Seq and microarrays. Just one fragment per transcript is produced and therefore there is no need for length normalization. This makes data analysis very simple and accurate. Up to 384 samples can be multiplexed in one lane, saving your sequencing space. QuantSeq is available for 3’ mRNA-Seq and targeted RNA-Seq.
QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina is the best solution for genome-wide gene expression analysis by sequencing towards the poly(A) tail.
New! QuantSeq 3’ mRNA-Seq V2 Library Prep Kits FWD with UDI 12 nt offer increased robustness, for enhanced performance on degraded samples and very low inputs. They naturally still include our state-of-the art, patented, Unique Dual Indices (also available separately).
QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina offers ultimate convenience for large screening projects. Using early pooling and batch processing it is easily scalable from a few to 36,864 samples. Combination of pooling (i1) indices with UDI gives you access to a triple indexing barcoding strategy, making sure that your libraries are correctly identified.
QuantSeq 3′ mRNA-Seq Library Prep Kit REV for Illumina is designed in a way that NGS reads start directly at the 3’ end of transcripts, enabling detailed 3’ UTR analysis and the study of alternative polyadenylation.
QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 is designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.
The CORALL RNA-Seq Library Prep Kits enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome analyses using Illumina® NGS platforms. CORALL is the universal solution for all your samples offering exceptional performance on low input samples, down to 1 ng starting amount. It is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples.
New! CORALL RNA-Seq V2 allows generation of longer libraries without RNA fragmentation for demanding applications such as alternative splicing, isoform analysis, and fusion detection.
New! CORALL mRNA-Seq V2 Kits provide a complete solution for mRNA sequencing and include poly(A) selection and library preparation to generate sequencing-ready whole transcriptome mRNA libraries in 5.5 hours. All CORALL mRNA-Seq Kits contain Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output.
New! CORALL Total RNA-Seq V2 Kits are the ideal choice for Total RNA-Seq with ribo-depletion and for degraded and FFPE samples. For convenience, CORALL Total RNA-Seq V2 Kits are available as bundled versions with RiboCop rRNA Depletion Kits. All CORALL Total RNA-Seq V2 Kits and Bundles contain Unique Dual Indices (UDIs) featuring superior error correction for maximal sequencing data output.
New! Luthor HD takes you into the world of high-definition single-cell RNA sequencing.
Why is high-definition single-cell RNA sequencing so important?
Most transcripts are present in low numbers in cells, typically between 1 and 10 copies. LUTHOR HD uses the innovative, state-of-the-art THOR amplification technology, which enables to capture and sequence even low-copy transcripts/genes, unraveling the full transcriptome of each cell (typically 95% of expressed genes at 1 M read depth). The THOR (T7 High-resolution Original RNA amplification) reaction allows amplification directly from the original mRNA rather than from cDNA, in contrast with conventional single-cell RNA-Seq protocols.
LUTHOR High-Definition (HD) is a 3′ mRNA sequencing kit and is intended mainly for gene expression profiling. LUTHOR HD is ideal when studying 1 to 100 cells or 10 pg to 1 ng of extracted total RNA. To analyze data stemming from sequenced LUTHOR HD libraries, a fast, simple, and affordable data analysis pipeline similar to the one used for our well-known QuantSeq.
Because only the endogenous polyadenylated RNA molecules are used as a template for the THOR reaction, LUTHOR HD does not require RNA extraction, mRNA enrichment, or ribosomal RNA depletion Likewise, it is practically insensitive to the presence of genomic DNA.
LUTHOR HD is provided as a standalone kit or with Lexogen’s patented 12 nt UDI (Set B). To minimize read loss and avoid misassignment, we highly recommend using our UDI, which you can demultiplex with our iDemux tool. You can also purchase any UDI from Set A separately with Cat. No. 101 to 104 and 156.
The TraPR Small RNA Isolation Kit isolates functional, physiologically relevant silencing sRNAs from any organism, tissue, cell type, or bio-fluid. In contrast to previous state-of-the-art methods, this innovative 15-minute single-column workflow neither requires prior knowledge of the sample, nor does it involve tedious gel extraction steps or lengthy immuno-precipitation procedures. TraPR thus enables the extraction of high-quality sRNAs even from challenging or uncharacterized material, leading to highly reproducible sequencing results.
The SPLIT RNA Extraction Kit enables fast and highly efficient extraction of high-quality, high-purity RNA from a broad range of biological samples, including cell culture, animal and plant tissue, and fluid samples. The obtained RNA is ideal for seamless library preparation for Next Generation Sequencing and other demanding applications such as full-length reverse transcription, sample preparation for microarray analysis, or RT-qPCR. SPLIT recovers the complete RNA size range, including small RNAs (<200 nt).
Lexogen offers single and dual indexing solutions for all needs. Most common solutions are included in all Lexogen’s library prep kits and additional variations are available as Add-on Kits.
384 Unique Dual Indices with superior error correction for maximal sequencing data output.
New! UDI V2 Add-on modules for increased robustness – same UDI sets, enhanced PCR mix. Also compatible with libraries from other vendors.
RiboCop rRNA Depletion Kits for Human/Mouse/Rat for Bacteria, and Yeast enable removal of ribosomal RNA from total RNA and are suited for Next Generation Sequencing as well as other demanding RNA analysis applications.
New! RiboCop for Yeast. Why limit yourself to mRNA? Unlock the complete yeast transcriptome and analyze non-coding RNAs with RiboCop rRNA Depletion.
New! Lexogen’s RNA/DNA Defender Solution is a non-toxic storage solution that stabilizes and protects fragile RNA molecules in fresh tissue and cell culture samples. It eliminates the need to process samples immediately or freeze them in liquid nitrogen for later processing.
TeloPrimeTM Full-Length cDNA Amplification Kit V2 generates full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. High-efficiency PCR produces excellent cDNA yields ideal for downstream long-read sequencing applications.
The SIRVs are available as sets of transcripts designed to validate the performance of RNA sequencing workflows and to control individual samples passing through RNA-Seq experiments. They are available in 3 sets.
SIRV-Set 1 (Iso Mix E0, E1, E2) is made for a detailed validation of isoform-specific RNA-seq pipelines.
SIRV-Set 2 (Iso Mix E0) enables the straightforward assessment and control of isoform detection and quantification in each sample.
SIRV-Set 3 (Iso Mix E0 / ERCC) that combines isoforms and non-isoform ERCC transcripts provides controls for examining isoform resolution and dynamic range resolution.
SIRV-Set 4 (Iso Mix E0 / ERCC / Long SIRVs) contains the long SIRV module with 15 RNAs of 4 – 12 kb length in addition to the 69 isoforms and 92 ERCC transcripts of SIRV-Set 3. SIRV-Set 4 covers three spike-in aspects: isoform complexity, abundance, and length.
What our clients say
“We have been using QuantSeq for over 5 years to profile samples that we have found previously difficult to target, such as formalin fixed, paraffin embedded (FFPE) samples. We have found that QuantSeq allows good reproducibility, low sample input requirements and rapid turnaround, allowing us to make the most of these precious samples.”
Andrew Beggs, Professor of Cancer Genetics & Surgery, Institute of Cancer and Genomic Sciences University of Birmingham, UK
“Transcriptional profiling is one of our key approaches to study and understand the molecular mechanism of action of cancer drugs. We have made excellent experiences with the CORALL kit both in terms of experimental feasibility but also data quality. Through integration with synthetic spike-ins SIRVs, It was particularly helpful when testing drugs that affect overall transcriptional output and thus cause a global decrease in mRNA levels.”
Georg Winter, PhD, Principal Investigator, CeMM, the Research Center for Molecular Medicine of the Austrian Academy of Sciences
“We have used the LUTHOR 3’ mRNA-Seq Library Prep Kit to profile single cells, which we FACS sorted before. The quality of the data is impressive: we detect high numbers of transcripts per cell by moderate sequencing depth. This allows us to comprehensively identify cell state and subtle cell state changes in the analyzed samples. For these aims, the 3’ mRNA-Seq library generated with the LUTHOR Kit has advantages over full-length cDNA approaches we have used previously. I can recommend LUTHOR for single-cell RNA-Seq analysis.”
Merrit Romeike, PhD Student, Buecker Group, Max Perutz Labs Vienna, Austria