miRVEL Discovery Small RNA-Seq Library Prep

Cutting-edge technology for sRNA discovery and biomarker studies

miRVEL Discovery Small RNA-Seq Library Prep Kit provides cutting-edge technology for sRNA-Seq, specifically optimized for low input biofluids, such as blood and plasma samples, making it a perfect solution for circulating biomarker studies. The miRVEL Discovery technology utilizes modified oligonucleotides that effectively inhibit the formation of adapter dimers and eliminate the need for gel-size selection, thereby reducing hands-on time and streamlining the overall workflow.

Unlock biofluid samples

Incorporation of hY4 Y RNA, which is frequently present at elevated levels in serum and plasma samples, is specifically inhibited, enabling more sequencing space on small RNAs of interest.

Precise quantification with UMIs

Unique molecular identifiers (UMIs) enable the removal of PCR duplicates, resulting in precise and unbiased quantification of sRNA.

10 nt UDIs

10 nt unique dual indices (UDIs) guarantee accurate read assignment, minimize the risk of read misassignment due to index hopping, and allow for multiplexing of up to 120 samples.

miRVEL Discovery Small RNA-Seq Library Prep provides a cutting-edge solution for sRNA-Seq with exceptional performance on low input biofluid samples.

Outstanding Reproducibility and Correlation Across Different Concentrations

miRVEL Discovery Small RNA-Seq Library Prep generates excellent gene count correlation between replicates (Fig. 1A, 1B) even with low inputs (1 ng plasma RNA, Fig. 1A). Moreover, the performance remains consistent across a range of inputs (Fig. 1C), which highlights the robustness of the miRVEL Discovery protocol.

Figure 1 | Correlation analyses of gene counts obtained from miRVEL Discovery Small RNA-Seq Library preps generated from 1 ng and 10 ng human plasma RNA. A) and B) Correlation between replicates. C) Correlation between 1 ng and 10 ng input RNA amount. The libraries were sequenced on NextSeq 2000 at 1 – 1.5 M read / sample (SR75).

Exceptional Rate of Reads Mapping in miRNAs for Biofluid Samples

miRVEL Discovery Small RNA-Seq Library Prep exhibits an exceptional rate of reads mapping to miRNA for libraries generated from 10 ng of human plasma RNA and 1 ng of human blood RNA (Fig. 2).

Figure 2 | Percentage of reads mapping to miRNAs. Libraries were prepared from 10 ng of human plasma RNA and 1 ng of human blood RNA with miRVEL Discovery Small RNA-Seq Library Prep and Competitor kit in duplicates. The obtained libraries were sequenced on NextSeq 2000 at 1 – 1.5 M read / sample (SR75). Lexogen’s miRVEL Discovery Small RNA-Seq showed an exceptional rate of reads mapping to miRNA compared to the Competitor.

Excellent miRNA Detection in Low-input Biofluid Samples

miRVEL Discovery Small RNA-Seq Library Prep demonstrates a high detection of miRNAs in low-input plasma samples (Fig. 3), making it an outstanding solution for biomarker research.

Figure 3 | Number of detected miRNAs in low-input plasma sample. Libraries were prepared from 1 ng of human plasma RNA with miRVEL Discovery Small RNA-Seq Library Prep kit and Competitor kit. The obtained libraries were sequenced on NextSeq 2000 at 1 – 1.5 M read / sample (SR75). Lexogen’s miRVEL Discovery Small RNA-Seq showed higher numbers of detected miRNAs at ≥ 5 reads than the competitor.

The miRVEL Discovery Small RNA-Seq Library Prep Kit features a streamlined workflow that allows the entire library preparation process to be completed in under 7 hours. The methodology utilizes modified oligonucleotides that effectively prevent adapter dimer formation, eliminating the need for time-consuming gel-size selection.

Step1:

RNA Input: total RNA extracted from tissue, cells, biofluids, exosomes, and other sample types. miRNAs and other small RNAs, such as siRNAs and piRNAs, possess 5' phosphate (5’P) and 3'hydroxyl (3’OH) groups, which allow for the specific ligation of adapters to both ends.

The input RNA is first subjected to 3’adapter ligation with a pre-adenylated DNA adapter.

5’ RNA adapter is attached to the 5′ end of the input RNA.

The input RNA, flanked by 5’ and 3’ adapters, is converted into cDNA using an RT primer that binds to the 3’ adapter. This RT primer includes a unique molecular identifier (UMI) that is incorporated into each cDNA molecule. Additionally, the RT primer introduces a universal sequence that is recognized by the sample indexing primers during library amplification.

cDNA libraries are produced and undergo bead-based purification.

During PCR library amplification, unique dual i7 and i5 indices, along with complete adapter sequences, are incorporated. The amplified libraries undergo a final bead-based purification.

A 72 bp single-read with 10 bp dual indexing is the recommended sequencing protocol. A 50 bp single-read protocol is adequate if UMIs are not required.

Frequently Asked Questions

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Please also check our General Guidelines and FAQ resources!

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User Guide – release 20.05.2025

Product Flyer – release 16.04.2025

miRVEL Discovery UDI-10-nt-Sequences – release 20.05.2025

Safety Data Sheet

Request Safety Data Sheet

If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.

Cat. No.	Product Name
242.24	miRVEL Discovery Small RNA-Seq Library Prep Kit, 24 preps
242.96	miRVEL Discovery Small RNA-Seq Library Prep Kit, 96 preps

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