Description

CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99 %). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites.

Features and Application:

CORALL is suitable for all whole transcriptome RNA-seq applications, including:

  • Gene expression profiling.
  • Isoform discovery and quantification.
  • Alternative splicing studies.
  • Transcript (re)annotation.
  • SLAMseq metabolic RNA sequencing.

System Compatibility:

  • HiSeq 2000/2500/3000/4000
  • NextSeq 500/550
  • MiSeq
  • NovaSeq6000

Kit Contents:

  • Reagent Box
  • Lexogen i7 6nt Index Set (indices 7001 – 7024, or 7001 – 7096) for 24 and 96 reaction kits, respectively
  • Purification Module with Magnetic Beads
  • User Guide

Data Analysis:

A Data Analysis Pipeline is now available on the BlueBee® Genomics Platform. The provided pipeline enables kit users to perform read quality control, mapping, Unique Molecular Identifier (UMI) deduplication, and transcript quantification.

Each Activation Code enables 24 data analysis runs (i.e., 24 fastq.gz files may be analyzed). Reference genomes for various species are available (please contact support@lexogen.com for more information).

Recommended! PCR Add-on Kit (96 rxn)

The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.