We are happy to be a Gold Sponsor of EMBL’s biannual Complex Life of RNA conference, which will be held virtually this year on October 7-9. Given the current pandemic situation, Lexogen is developing and providing essential products and services for SARS-CoV-2 research and testing. However, while impacted by restrictions and lockdowns, research in other RNA areas fortunately does not stop, and as a true transcriptomics company Lexogen continuously offers and develops a comprehensive R&D-driven portfolio to the general RNA research community.
Lexogen is therefore looking forward to getting in touch with the participants of “Complex Life of RNA” and introducing two new products that were launched recently.
Join our virtual events:
Lexogen Pre-Conference Webinar:
In this pre-conference webinar on October 6 (4 – 5 pm CEST) Lexogen’s Research Scientist, Dr. Yvonne Goepel, will give a talk “Purifying functional small RNAs in 15 minutes using the universal, column-based TraPR method”. In this talk you will be introduced to a functional way of purifying sRNAs with the Lexogen TraPR Small RNA Isolation Kit, also from low input and degradation-prone samples, using a straight-forward, centrifugation-based procedure.
Find the ZOOM link to for our Pre-Conference webinar HERE.
Password for the meeting: lexogen
Purifying functional small RNAs in 15 minutes using the universal, column-based TraPR method
Dr. Yvonne Goepel
Research Scientist, Lexogen
Regulatory small RNAs (sRNAs) play an essential role in mRNA turnover, translational regulation, and chromatin compaction and important regulators of gene expression. These sRNAs associate with specific proteins of the Argonaute family (AGOs) to form RNA-induced silencing complexes (RISCs) and guide the AGO proteins to their respective targets in a process termed RNA interference.
Lexogen’s TraPR Small RNA Isolation Kit greatly facilitates the isolation of RISCs and associated small functional RNAs. It is based on TraPR (Trans-kingdom, rapid, affordable Purification of RISCs), a gel- and bias-free, column-based method (Grentzinger et al., 2020). In a straightforward workflow, freshly harvested or flash frozen tissue or cells are lysed and loaded directly onto TraPR columns. While bulk RNA and DNA are retained on the column, only RISC-associated sRNAs are eluted. These steps are completed in 15 minutes, and the resulting RISC fraction can be analyzed directly or sRNAs can be extracted for further analyses including small RNA sequencing (sRNA-Seq).
Whereas other methods for functional sRNA isolation such as co-immunoprecipitation need species-specific antibodies, TraPR does not require any prior characterization of the sample. Further, by purification of RISCs the TraPR Small RNA Isolation Kit enriches exclusively fully functional, physiologically relevant sRNAs including piRNAs, siRNAs, miRNAs, and scnRNAs. Contaminating RNAs such as degradation products of tRNA, rRNA, and mRNA are effectively excluded from the purified RISC fraction. TraPR is thereby superior to methods that are based on size-selection via columns or gel-purification and cannot distinguish between functional small RNAs and degradation products.
We show, in multiple benchmarking assays, that the TraPR kit considerably improves the quality and consistency of silencing sRNA sample preparation including from notoriously difficult-to-handle tissues/bio-fluids such as starchy storage roots or mammalian plasma, and regardless of RNA contaminants or RNA degradation status of samples. TraPR works consistently over a large input range and eliminates the need for species-specific rRNA depletion (e.g. 2S rRNA in Drosophila).
In summary, the TraPR Small RNA Isolation Kit generates high-quality sRNA preparations suitable for Next Generation Sequencing (NGS) applications and thus provides a highly reproducible, time-saving method that outperforms all current gold-standard procedures for sRNA profiling. Small RNA isolation from liquid samples becomes easily accessible, making TraPR perfectly suited for biomarker discovery applications.
Grentzinger, Thomas; Oberlin, Stefan; Schott, Gregory; Handler, Dominik et al. (2020): A universal method for the rapid isolation of all known classes of functional silencing small RNAs. Nucleic Acids Research. DOI: 10.1093/nar/gkaa472.
Watch the recording
In the 10 min. pre-recorded talk, Lexogen’s Senior Manager of Scientific Affairs, Lukas Paul, PhD, will present the addition of long spike-in transcripts with up to 12 kb (!) in length to Lexogen’s external RNA-seq controls portfolio. Value for RNA-Seq experiments, modular structure, and technical details of the Spike-In RNA Variants (SIRVs) will be highlighted.
The talk recording will be accessible at EMBL Media site for 1 week after the start of the meeting. The access link and individualized login information will be sent by the organizers to the registered attendees before the conference. It is recommended to use Chrome as a preferred browser.
Lexogen Live Q&A Session:
We will be available to answer your questions about the talks as well as our products and services in a LIVE Q&A session on Thursday, October 8. Our speakers (Senior Manager of Scientific Affairs, Lukas Paul, PhD and Research Scientist, Dr. Yvonne Goepel) will be available for 20 minutes at 7.30 pm – 7.50 pm CEST.
Find the ZOOM link for the Q&A HERE.
You can also leave your question with us by e-mailing it to Lukas Paul – email@example.com and we will make sure they are answered in the Q&A session.