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Lexogen Indexing Solutions

Lexogen’s Indexing Solutions

Information:

Single indexing = one index (i7) per library
Dual indexing:
  • combinatorial (also called CDI for Combinatorial Dual Indexing) = two indices (i5 and i7) on each end of the insert, in different combinations. Each i5 and each i7 can be used several times (but each combination is unique).
  • unique (also called UDI for Unique Dual Indexing) = two indices (i5 and i7) on each end of the insert, in different combinations. Each i5 and i7 is unique (you will never find the same i5 or i7 index twice in a pair).
Lexogen_RNA-LEXICON_Chapter9_Graph-04

Lexogen offers single indexing, dual indexing and unique dual indexing for all needs.

Prevalent indexing solutions as part of Lexogen’s library prep kits:

QuantSeqCORALL
Indexingrxni7 6 nti5 6ntUDI 12ntCat. No.i7 6ntUDI 12ntCat. No.
Single2424015.2424095.24
9696015.9696095.96
1922x96015.2x96
DualCombinatorial384964015.384
Unique V2
(recommended)
2424191.24 (A1), 192.24 (B1)171.24 (A1), 175.24 (B1)
9696191.96 (A1), 192.96 (B1), 194.96 (A2), 195.96 (A3), 196.96 (A4)171.96 (A1), 175.96 (B1), 172.96 (A2), 173.96 (A3), 174.96 (A4)
384384193.384176.384
Unique V19696113.96 (A1), 115.96 (B1), 129.96 (A2), 130.96 (A3), 131.96 (A4)96117.96 (A1), 118.96 (B1), 132.96 (A2), 133.96 (A3), 134.96 (A4)
384384115.384384119.384

Flexible dual indexing solutions available as Add-on kits to be combined with library prep kits:

Number of indices includedCat. No.
IndexingPossible index combinationsi7 6nt
(included in Lexogen’s library prep kits)
i5 6nt
(Add-on kits)
UDI 12nt
(Add-on kits)
i5 6nt Dual Indexing Add-on Kit312nt UDI Add-on Kit3
(recommended)
QuantSeq
(i7 6nt indices included)
CORALL
(i7 6nt indices included)
Dual
(combinatorial)
96244047.4x96¹015.24095.24
384964047.4x96¹015.96095.96
9,2169696047.96015.96095.96
Unique Dual969696047.96015.96095.96
96(96)²96 pairs198.96 (A1), 199.96 (A2), 200.96 (A3), 201.96 (A4), 202.96 (B1)015.96095.96
384(96)²384 pairs203.384015.384⁴4x 095.96

Notes:
1 module 047.4×96 provides enough i5 primers for 4×96 reactions
2 these primers will be discarded, and replaced with UDI
3 the PCR mix of the Add-on kit must be used instead of the one provided in the QuantSeq or CORALL kit
4 i5 indices will be discarded, and replaced with UDI

The Indexing Add-on kits are further described below.

Unique Dual Indexing with 8, 10, or 12 nt read-out (recommended)

  • Lexogen UDI 12nt UDI Add-on V2 Kits for unique dual indexing of up to 96 (Cat. No. 198.96 to 202.96) and 384 (Cat. No. 203.384) samples, with improved robustness. These kits are compatible with existing CORALL and QuantSeq kits, but also library preparation kits from other vendors.
  • The V1 Add-on kits (107.96, 108.96, 109.96, 110.96, 111.96 and 120.384) are still available for purchase for a limited period
  • Add-on kits include the PCR amplification mastermix (UDI Sets are available as a standalone product with Cat. No. 101-105 and 156)

Dual Indexing with 6 nt read-out

To be used with the i7 6 nt Index Set provided in the library preparation kit (QuantSeq FWD 015 or CORALL 095)

  • Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) for dual indexing of up to 96 (4×24) samples (Cat. No. 047.4×24)
  • Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) for dual indexing of up to 384 (4×96) samples (Cat. No. 047.4×96)
  • Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096) for dual indexing of up to 9,216 (96×96) samples* (Cat. No. 047.96)

Unique Dual Indexing with 6 nt read-out

To be used with the i7 6 nt Index Set provided in the library preparation kit (QuantSeq FWD 015 or CORALL 095)
  • Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096) for unique dual indexing of up to 96 samples (Cat. No. 047.96)

Single Indexing with 6 nt read-out

These indices are already provided in QuantSeq and CORALL kits 015 and 095. They can be purchased separately for other purposes, or as a replacement set.

  • Lexogen i7 6 nt Index Set (7001-7096) for single indexing of up to 96 samples (Cat. No. 044.96)

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