Get the most out of your data with the latest updates for CORALL Total RNA-Seq

Yvonne Goepel, Research Scientist (Kit Development)

Common Next Generation Sequencing (NGS) library preparations depend on fragmentation which may cause difficulties during transcript quantification. CORALL is a fast and highly reliable total RNA library preparation method with inherent strand-specificity that is free from fragmentation bias and allows accurate transcript expression profiling. Integration of unique molecular identifiers (UMIs) and CORALL’s exceptional accuracy at transcriptional start and end sites improve isoform identification and quantification. After release a year ago, Lexogen has added unique features for customer convenience, including a scalable 12-nt unique dual indexing (UDI) system with superior error correction and validated data analysis workflows that are easily accessible on commonly used platforms.

New RiboCop rRNA Depletion Kit for Bacteria

Alex Mojcher, Application Scientist

The current landscape of NGS technology is regularly utilized for RNA-Seq methods to explore bacterial transcriptomics. Lexogen has developed a highly effective ribosomal depletion kit for bacteria using an affinity probe method. Bacterial RNA consists of up to 98% ribosomal content that must be depleted ahead of transcriptomic analysis. Lexogen’s Ribocop rRNA Depletion Kits for Bacteria remove 23S, 16S, and 5S rRNA from both monocultures and mixed bacterial samples ranging from 1 ng to 1 µg total RNA. Input RNA from intact as well as degraded material may be used, making this a powerful tool for studying the bacterial transcriptome.