miRVEL Profiling Small RNA-Seq Library Prep
The miRVEL Profiling Small RNA-Seq Library Prep Kit provides the ideal solution for sRNA profiling studies with unique dual indices (UDIs). The miRVEL Profiling technology effectively prevents the formation of adapter dimers through adapter blocking, eliminates purification steps prior to library amplification and the need for gel-based size selection for a fast and streamlined sRNA-Seq workflow.
Benefits
Time Saving
Cost-efficient
Excellent reproducibility
Performance
Exceptional Reproducibility Across Replicates and Input Amounts
miRVEL Profiling Small RNA-Seq generates excellent gene count correlation between replicates at low read depth of 1 M reads / sample (Fig. 1A, 1B). High correlations are achieved even for low inputs of 10 ng total RNA from human brain (Fig. 1 B) and across a range of inputs highlighting the robustness of the miRVEL Profiling protocol (Fig. 1C).
miRNA Mapping Rates across Different Input Amounts
miRVEL Profiling Small RNA-Seq exhibits robust mapping rates to miRNA for libraries generated from 10 ng or 100 ng of human brain RNA (Fig. 2).
Figure 2 | Percentage of reads mapping to miRNAs. Libraries were prepared in triplicates from 100 ng and 10 ng of human brain RNA using the miRVEL Profiling Small RNA-Seq Library Prep. The obtained libraries were sequenced on NovoSeq X and downsampled to 1 M read / sample.
Excellent miRNA Detection from Human Brain RNA
miRVEL Profiling Small RNA-Seq demonstrates a high detection of miRNAs across different inputs of human brain RNA even at low sequencing depth of 1 M reads / sample (Fig. 3).
Workflow
FAQ
Frequently Asked Questions
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Downloads
Safety Data Sheet
If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.
Ordering Information
| Cat. No. | Product Name |
| 243.24 | miRVEL Profiling Small RNA-Seq Library Preparation Kit, 24 preps |
| 243.96 | miRVEL Profiling Small RNA-Seq Library Preparation Kit, 96 preps |
| Associated Product | |
| 246.96 | miRVEL Profiling PCR Add-on and Reamplification Kit, 96 rxn |
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