Ultra-low Input RNA Sequencing
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Ultra-low Input RNA Sequencing
Ultra-low input RNA-Seq enables insight into gene expression profiles even when dealing with very low inputs. This is often the case when working with rare cell types, having access to a limited amount of material, or being interested in lowly abundant transcripts present only in a small population of cells. We have substantial experience making low and ultra-low input RNA-Seq work and providing accurate and reliable transcriptomic datasets from these limited samples. Our proprietary, high-definition technology enables us to work with RNA input as low as 10 pg and achieve unprecedented sensitivity and reproducibility, even with low-quality samples.
Benefits
- Ultra-low input: 10 pg to 1 ng of total RNA*, corresponding to 1-100 cells.
- Compatible with cell lysates
- Supreme sensitivity results in more detected genes than other high-throughput technologies.
- Detection of lowly expressed transcripts (<10 copies/cell) possible
- We also accept low-quality RNA
*Our technology enables us to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts.
Applications
- High-definition single-cell differential gene expression analysis
- Extraction-free high-throughput RNA-Seq (DRUG-Seq)
- Post-bulk scRNA-Seq characterization of cell subpopulations
- Gene expression analysis of rare cell types or cells that are scarce, such as circulating tumor cells (CTCs) or innate lymphoid cells (ILCs)
- Subcellular (cytoplasm, organelle) RNA analysis
- Single nucleus RNA-Seq
- Gene expression analysis of low abundant transcript analysis
Deliverables
- Raw data
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts + counting summary (.tsv)
- Differential expression + DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- Deliverables summarized in a PDF report
Working with us
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
- Shipping ultra-low input RNA samples requires our RNA expert input.
Sample Preparation
- Sorted and lysed cells, or purified, ultra-low input RNA as starting material.
- Low-quality and challenging samples, e.g., FFPE material, possible.
- Sample QC included (where possible).
Library Preparation
- Library QC included.
- Using proprietary Lexogen technologies, adapted to your needs.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
