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Gene Expression Profiling
RNA sequencing is a powerful technique for studying gene expression. To simply quantify differentially expressed genes between conditions or samples, 3′ mRNA sequencing is a cost-effective and efficient choice. For a comprehensive analysis, including detection of isoforms, single nucleotide polymorphisms (SNPs), and splicing variants, full-length mRNA sequencing is the preferred method. Selecting the appropriate RNA-Seq approach is essential for obtaining reliable and meaningful results.
Benefits
- Cost-efficient gene expression analysis
- Focus on polyadenylated transcripts
- Flexible offering suited to data requirements
- Ideal for low-input material
- Compatible workflow for low-quality RNA
Applications
- Differential gene expression studies
- Isoform and alternative splicing pattern identification of mRNAs
- High-throughput gene expression sequencing, e.g., for compound screening
- Drug discovery & development studies, e.g., with High-throughput kinetic RNA-Seq (after SLAMseq)
- FFPE Transcriptomics
- Whole Blood Transcriptomics
- Plant Transcriptomics
- Targeted RNA sequencing
- Determination of alternative poly(A) sites
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts and counting summary (.tsv)
- Differential expression and DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are accepted. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
