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Blood RNA Sequencing
Blood transcriptomics provide valuable insights into the expression and abundance of all transcripts on a system-wide scale. The development of high-throughput RNA-Seq technologies accelerates the discovery of biomarkers and revolutionizes our understanding of drug responses, pathological and physiological processes, and the non-invasive diagnosis of diseases.
Lexogen’s Blood RNA-Seq Services provide customizable transcriptome analysis of whole blood samples and other biofluids, including nucleic acid extraction from standarized blood collection tubes, whole transcriptome sequencing, large-scale gene expression studies, and combined DNA sequencing of blood samples.
Benefits
- Years of hands-on experience with degraded and heterogenous blood RNA
- Compatible with low RNA input blood material
- Direct RNA extraction from blood collection tubes
- Well-established workflows for Blood RNA-Seq
- Ideal for large scale high-throughput screening projects
Applications
- High-throughput gene expression sequencing from human blood
- Whole Transcriptome Sequencing of blood samples, e.g., for detection of fusion genes, isoforms, SNPs etc.
- Analysis of non-coding transcripts, including lncRNA, snRNA, snoRNA, circRNA, etc.
- Drug discovery & development studies, e.g., target validation, biomarker identification
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts and counting summary (.tsv)
- Differential expression and DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us blood samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep).It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are accepted. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- Whole blood RNA extraction from PAXgene®, Tempus™ or other blood collection tubes.
- RNA extraction from serum, plasma, PBCMs etc.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
