How QuantSeq Works

Interested to Try QuantSeq?

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What QuantSeq Can Do for You

QuantSeq is a well-established 5-step mRNA-Seq library prep, finished in only 4.5 hours, and enabling highly multiplexed NGS experiments – even with FFPE samples!

Lexogen-QuantSeq-Icon_Time saving

Time saving

Time saving

QuantSeq offers a fast and fully automatable 3’ mRNA-Seq workflow with half as many pipetting steps as conventional mRNA-Seq library preps.

Lexogen-QuantSeq-Icon_Cost-efficient

Cost saving

Cost saving

QuantSeq generates only one read per transcript freeing up sequencing depth to multiplex more samples per lane, ultimately lowering sequencing cost per sample.

Lexogen-QuantSeq-Icon_Well-established

Proven technology

Proven technology

QuantSeq simplifies RNA-Seq projects all over the world and was used in over 900 publications.

Challenging samples

Challenging samples

QuantSeq’s robust protocol enables expression profiling of challenging samples types, such as FFPE-derived or degraded RNA, including RNA from biofluids.

Facts about QuantSeq

4.5 hrs

Protocol time

RNA-Seq library prep in only 4.5 hours from RNA to ready-to-sequence library

>900

Publications

Hundreds of publications citing the successful use of QuantSeq

36,864 samples

Samples per run

Multiplexing of up to 36,864 samples possible with QuantSeq-Pool

4,601

Saved pipet tips

20x less consumables with QuantSeq-Pool than with conventional mRNA-Seq

QuantSeq Compared to Other mRNA-Seq Methods

QuantSeq is completed in only 5 steps and does not require any RNA pre-processing such as mRNA selection. Thus, QuantSeq offers an accelerated workflow in comparison to conventional mRNA-Seq methods and requires only half the hands-on steps (26 pipetting steps for QuantSeq vs. >50 for conventional mRNA-Seq library preparation), saving valuable time and consumables. The streamlined workflow also renders QuantSeq highly robust and less sensitive to handling variations.

QuantSeq intro - Comparison Graphic

Technical Details

For more technical details about QuantSeq performance and applications, please visit our QuantSeq product page.