How QuantSeq Works
Interested to Try QuantSeq?
First Time User? We’re excited to offer you an exclusive introductory offer.
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What QuantSeq Can Do for You
QuantSeq is a well-established 5-step mRNA-Seq library prep, finished in only 4.5 hours, and enabling highly multiplexed NGS experiments – even with FFPE samples!
![Lexogen-QuantSeq-Icon_Time saving](https://www.lexogen.com/wp-content/uploads/2021/05/Lexogen-QuantSeq-Icon_Time-saving-150x150.png)
Time saving
QuantSeq offers a fast and fully automatable 3’ mRNA-Seq workflow with half as many pipetting steps as conventional mRNA-Seq library preps.
![Lexogen-QuantSeq-Icon_Cost-efficient](https://www.lexogen.com/wp-content/uploads/2021/05/Lexogen-QuantSeq-Icon_Cost-efficient-150x150.png)
Cost saving
QuantSeq generates only one read per transcript freeing up sequencing depth to multiplex more samples per lane, ultimately lowering sequencing cost per sample.
![Lexogen-QuantSeq-Icon_Well-established](https://www.lexogen.com/wp-content/uploads/2021/05/Lexogen-QuantSeq-Icon_Well-established-150x150.png)
Proven technology
QuantSeq simplifies RNA-Seq projects all over the world and was used in over 900 publications.
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Challenging samples
QuantSeq’s robust protocol enables expression profiling of challenging samples types, such as FFPE-derived or degraded RNA, including RNA from biofluids.
Facts about QuantSeq
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Protocol Time
RNA-Seq library prep in only 4.5 hours from RNA to ready-to-sequence library
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Publications
Hundreds of publications citing the successful use of QuantSeq
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Samples per run
Multiplexing of up to 36,864 samples possible with QuantSeq-Pool
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Saved pipet tips
20x less consumables with QuantSeq-Pool than with conventional mRNA-Seq
QuantSeq Compared to Other mRNA-Seq Methods
QuantSeq is completed in only 5 steps and does not require any RNA pre-processing such as mRNA selection. Thus, QuantSeq offers an accelerated workflow in comparison to conventional mRNA-Seq methods and requires only half the hands-on steps (26 pipetting steps for QuantSeq vs. >50 for conventional mRNA-Seq library preparation), saving valuable time and consumables. The streamlined workflow also renders QuantSeq highly robust and less sensitive to handling variations.
![QuantSeq intro - Comparison Graphic](https://www.lexogen.com/wp-content/uploads/2021/05/QuantSeq-intro-Comparison-Graphic.jpg)
Technical Details
For more technical details about QuantSeq performance and applications, please visit our QuantSeq product page.